HiDef® B8 stem cell growth medium Price range: £110.00 through £1,255.00View product
HiDef® B8 stem cell growth medium
Price range: £110.00 through £1,255.00
Defined Bioscience’s HiDef® B8 is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state.
To better support small-scale experiments and pilot studies, the HiDef® B8 400x Supplement is offered in two vial sizes. Standard vials prepare 500 ml of complete medium, while miniature vials prepare 50 ml, enabling convenient preparation of smaller volumes without excess waste.
HiDef® B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGF-β3, NRG-1, and thermostable FGF-2/bFGF. The addition of further components is not required.
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Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £110.00 through £1,255.00
1000 µg will be dispatched as 2 x 500 µg.
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Summary:
Fully defined
Convenient use
Weekend-free
Tested across >100 hiPSC and hESC lines
Animal origin free (AOF) product
- Product supplied in liquid form
Featured applications:
Induced pluripotent stem cell culture and maintenance
Alternative products
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Product background
HiDef® B8 from Defined Bioscience is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state. The HiDef-B8 medium is a unique formulation that offers the modern conveniences of a flexible feeding schedule (including weekend-free maintenance) and the ability to choose the matrices and passaging methods that best suit specific applications.
Key characteristics
HiDef® B8 complete medium is a specially formulated defined medium that maintains human pluripotent stem cells in feeder-free and serum-free conditions with reduced hands-on cell culture effort and lower frequency feeding required.
- Feeder-free, serum-free, defined cell culture medium for human PSCs
- Versatile – allows enzyme-free passaging from multiple substrates and supports enzymatic single-cell passaging
- Supports the stable expansion and maintenance of PSC lines for >50 passages without karyotypic abnormalities
- Less frequent cell feeding eliminates the requirement for weekend labor. Take the weekend off!
Preparation of complete HiDef® B8 medium
Use appropriate aseptic technique to prepare HiDef® B8 complete medium (DMEM/F12 basal medium + HiDef-B8 400x Supplement). The following example is for preparing 501.25 ml of HiDef-B8 complete medium. If preparing other volumes, adjust accordingly.
NOTE: Thaw supplements or complete medium at room temperature (15 – 25°C) and use immediately. Do not thaw in a 37°C water bath, overnight at 4°C, or on ice.
- Thaw HiDef® B8 400x Supplement and warm to room temperature. Mix thoroughly via inversion or gentle pipetting. It is normal to observe turbidity after thaw. Immediate dissolution should still be observed when added to basal medium (Step 2).
- Add 1.25 ml of HiDef® B8 400x Supplement (the full volume provided by the manufacturer) to 500 ml of DMEM/F12. Mix thoroughly and avoid foaming.
- If prepared aseptically, HiDef® B8 complete medium is ready for use. Store complete HiDef® B8 medium at 2 – 8°C for two weeks. Once prepared, do not freeze/thaw HiDef® B8 complete medium.
Additional resources
FAQ
What is HiDef® B8?
HiDef® B8 is a complete, serum-free, feeder-free medium for human pluripotent stem cells.
What is unique about HiDef® B8?
HiDef® B8 maintains PSCs for >50 passages without karyotypic abnormalities, supports weekend-free feeding, and is compatible with multiple matrices and passaging methods.
Can I switch from other media to HiDef® B8 directly?
Yes. PSCs can be transitioned to HiDef-B8 either 1. directly or 2. by gradual adaptation, in active cell cultures or at time of thawing. Please see our transition guide for further details.
How is HiDef® B8 stored?
Store the 400x supplement at -20°C; prepared complete medium can be kept at 2–8°C for up to 2 weeks.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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