Food grade Beefy9 media growth factor discovery kit (Qk504-FG)

Kit description

Find the recipe for Beefy-9 here.

The innovation of adding recombinant albumin to B8 media has yielded a ground-breaking advancement in cell culture for the growth of bovine satellite cells, resulting in the development of Beefy-9 media [1-2]. This novel medium is the first of its kind, meeting fundamental criteria to serve as a foundational medium for cultured meat production. One key feature includes the complete absence of animal-derived components, ensuring animal-component-free culture conditions. Even, vitronectin, crucial for cell adhesion to culture flasks, is produced without animal inputs. This departure from traditional serum-containing media, which often rely on animal-derived additives, distinguishes the use of Beefy-9 in the commitment to ethical and sustainable practices in cell culture [1,3-4].

Beefy-9 incorporates food-safe components, excluding any chemicals or compounds, such as synthetic steroids, that could potentially raise regulatory concerns regarding food safety. This feature enhances confidence in the safety and suitability of cultured meat products developed using Beefy-9 [1,5].

The transparency of the recipe for Beefy-9 distinguishes it from previous serum-free media options. Its fully described and defined composition, devoid of proprietary ingredients, facilitates reproducibility and allows for easy modification and optimization. Laboratories interested in advancing cultured meat research can readily adopt and build upon Beefy-9 without facing hurdles related to complex formulations or proprietary restrictions [6].

It is generally accepted that best practice is to use growth factors from the target species for reasons of consumer acceptance, regulatory considerations and species-matched activity including differences in receptor binding and efficacy in some cases. Some growth factor protein sequences are highly conserved and 100% identical across species. There is currently a lack of commercial availability of cultured-meat and fish relevant species-matched growth factors, so often human proteins, or indeed bovine serum, are used as a surrogate. It’s advisable to determine the conservation of protein sequence when selecting appropriate growth factors and switch to species-matched proteins if feasible.

Qkine provide extensive quality analysis, documentation and support with novel food dossier preparation. Qkine food grade growth factors are manufactured in the UK under a food manufacturing HACCP regime. Full raw materials traceability, allergen analysis, CoO, CoA, beta-lactam-free and animal origin-free certification are available.

Qkine high quality food grade products are intended solely for use as food processing aids, ex vivo cell manufacturing and research use. Not for direct human consumption, therapeutic or diagnostic use.

The authorization of novel foods, including the use of growth factors as food processing aids, is regulated by regional government agencies. The use of our products as food processing aids in novel foods requires the end-user to obtain the necessary regulatory approvals.

Additional information