Technote | BMP-4 (Qk038)
Qkine bone morphogenetic protein 4 (BMP-4) protein produces consistently high bioactivity lot-to-lot
Human bone morphogenetic protein 4 (BMP-4) is a key regulator of embryogenesis and supports the differentiation of induced pluripotent stem cells (iPSC) and embryonic stem cells (ECS). BMP-4 plays a role in bone and cartilage formation, tissue repair, and organ and neuronal development.
BMP-4 is part of the TGF-β superfamily and has a complex biochemical structure, expertise is required to manufacture bioactive BMP-4. Qkine BMP-4 (Qk038) has exceptional lot-to-lot consistency.
Qkine BMP-4 lot validation
- Four independent lots of BMP-4 (Qk038) were tested using a BMP-4 responsive firefly luciferase reporter assay.
- Qkine recombinant human BMP-4 lots show a narrow bioactivity range across the four batches of 17-34 pM.
Qkine BMP-4 (Qk038) protein has exceptional lot-to-lot consistency. Bioactivity was determined using a BMP-4-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of four independent lots of BMP-4 for 6 hours in triplicate.
| Lot | EC50 (pM) |
|---|---|
| #104296 | 33.1 |
| #104329 | 18.3 |
| #104357 | 17.2 |
| #104357 | 29.0 |
Qkine BMP-4 (Qk038) is highly pure, bioactive and animal origin-free. Qkine tests all newly manufactured lots against previous lots ensuring exceptional lot-to-lot consistency and allowing increased reproducibility for stem cell culture and differentiation.
