Technote | TNF-α (Qk083)
Qkine tumor necrosis factor-alpha (TNF-α) is as biologically active as a comparable alternative supplier protein
Tumor necrosis factor-alpha (TNF-α) is a cytokine involved in cell signaling, primarily produced by immune cells like macrophages.
Qkine recombinant TNF-alpha is animal origin-free, carrier-free and protein tag-free to ensure its purity and is suitable for the culture of reproducible and high-quality hematopoietic stem cells and other relevant cells.
Qkine TNF-alpha bioactivity
- Qkine TNF-alpha (Qk083) was bioactive in a quantitative assay with an EC50 of 1.5 ng/ml (87 pM).
- This was slightly more bioactive than TNF-alpha from an alternative supplier, which had an EC50 of 2.5 ng/ml (145 pM).
Qkine TNF-α had comparable bioactivity to a alternative supplier protein. Bioactivity was determined using a NF-κB firefly luciferase reporter assay. Transfected HEK293T cells were treated in triplicate with a serial dilution of Qkine TNF-α (Qk083, green) or Peprotech TNF-α (300-01A, Supplier B, black) for 3 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk083 #204609.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of hematopoietic stem cells.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.