Technote | KGF/FGF-7 (Qk046)
Qkine keratinocyte growth factor (KGF)/fibroblast growth factor 7 (FGF-7) bioactivity is comparable to an alternative supplier protein
Human KGF (FGF-7) protein is a member of the FGF family. KGF protein is used in the culture of lung, breast, liver, and bladder organoids.
Qkine KGF (FGF-7, Qk046) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine KGF (FGF-7) bioactivity
- Qkine KGF (FGF-7) was bioactive in a quantitative luciferase assay with an EC50 of 2.0 ng/ml.
- This was comparable to KGF from an alternative supplier, which had an EC50 of 3.7 ng/ml.
Qkine KGF was as bioactive as KGF from an alternative major supplier. Bioactivity was determined using the Promega serum response element (SRE) luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of Qkine KGF (Qk046, green) or Peprotech KGF (100-19, Supplier B, black) for 4 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. Data from Qk046 lot #104425.
Qkine recombinant proteins are highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.