Technote | FGF-10 (Qk003)
Qkine Fibroblast Growth Factor 10 (FGF-10) is as biologically active as a comparable alternative supplier protein
Fibroblast growth factor 10 (FGF-10) is involved in several different embryo and adult cell and tissue types, including mesenchymal, neuronal and epithelial cells. FGF-10 is used widely for stimulating the differentiation of induced pluripotent stem cells (iPSC) and embryonic stem cells (ESC) and promoting organoid formation.
Qkine FGF-10 (Qk003) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine FGF-10 (Qk003) bioactivity
- Qkine FGF-10 (Qk003) was bioactive in a quantitative luciferase assay with an EC50 of 1.32 ng/ml (77.6 pM).
- This was comparable to FGF-10 from an alternative supplier, which had an EC50 of 1.48 ng/ml (87 pM).
Qkine FGF-10 was as bioactive as FGF-10 from an alternative major supplier. Bioactivity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of Qkine FGF10 (Qk003, green) or Peprotech FGF-10 (100-26-25UG, Supplier B, black) for 4 hours. Protein concentrations were measured before use (Peprotech vial recovery 93%, Qkine vial recovery 109%). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
This bioactivity comparison demonstrates that Qkine FGF-10 (Qk003) has equivalent bioactivity to FGF-10 from an alternative major supplier. Qkine FGF-10 has the advantage of being highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of stem and organoids.
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