Technote | BDNF (Qk050)
Qkine human BDNF is as biologically active as a comparable alternative supplier protein
Recombinant BDNF is used to maintain neurons and differentiate and mature human pluripotent stem cell-derived neural progenitors to cortical and motor neurons and cortical organoids.
Qkine human BDNF (Qk050) is animal origin-free, carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency.
Qkine BDNF bioactivity
- Qkine BDNF (Qk050) was bioactive in a quantitative luciferase assay with an EC50 of 0.42 ng/ml.
- This bioactivity was slightly higher than BDNF from an alternative supplier, which had an EC50 of 0.96 ng/ml.
Qkine BDNF was as bioactive as BDNF protein from an alternative supplier. Recombinant BDNF activity was determined using a NFAT-luciferase reporter assay in transfected HEK293T cells also expressing TrkB. Cells are treated in triplicate with a serial dilution of Qkine BDNF (Qk050, green) or Peprotech BDNF (Supplier B, black) for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk050 lot #104465.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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