Technote | Activin A (Qk001)
Qkine activin A is as biologically active as a comparable alternative supplier protein
Activin A is frequently used to maintain pluripotency in induced pluripotent and embryonic stem cell cultures. It is also used in many stem cell differentiation protocols, including endoderm lineage differentiation and further maturation into hepatocyte and pancreatic cells.Â
Qkine activin A (Qk001) is a high-purity mature bioactive dimer which is animal origin-free, carrier protein-free and tag-free.
Qkine activin A bioactivity
- Qkine activin A (Qk001) was bioactive in a quantitative luciferase assay with an EC50 of 16 pM.
- This was comparable to bioactivity of activin A from an alternative supplier, which had an EC50 of 12 pM.
Qkine activin A was as bioactive as activin A protein from an alternative supplier. Activin A activity was determined using the activin-responsive firefly luciferase reporter assay in transiently transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine activin A (Qk001, green) or Peprotech activin A (AF-120-14E, black). Protein concentrations were measured before use (Peprotech vial recovery 84%, Qkine 102%). Firefly luciferase activity was measured and normalized. Data from Qk001 lot #204536.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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