Technote | Activin A (Qk005)
Qkine activin A PLUS™ is as biologically active as activin A protein from an alternative supplier
Activin A PLUS™ protein is an optimized biologically active truncation of the mature domain of human activin A protein. The EC50 and activity in stem cell culture of activin A PLUS™ is equivalent to the full mature domain activin A.
Qkine activin A PLUS™ (Qk005) is high-purity, animal origin-free, carrier protein-free and tag-free.
Qkine activin A PLUS bioactivity
- Qkine activin A PLUS™ (Qk005) was bioactive in a quantitative luciferase assay with an EC50 of 6.9 pM.
- This was comparable to bioactivity of activin A from an alternative supplier, which had an EC50 of 7.9 pM.
Qkine activin A PLUS™ was as bioactive as activin A protein from an alternative supplier. Activin A activity was determined using the activin-responsive firefly luciferase reporter assay in transiently transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine activin A PLUS™ (Qk005, green) or Peprotech activin A (AF-120-14E, black). Protein concentrations were measured before use (Peprotech vial recovery 84%, Qkine 102%). Firefly luciferase activity was measured and normalized. Data from Qk005 lot #104344.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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