Technote | TGF-ß (Qk010)
Qkine animal origin-free TGF-β1 PLUS™ is more bioactive than mammalian expressed alternatives
Transforming growth factor beta-1 (TGF-β1) is a common component of human pluripotency maintenance media. However, as TGF-β1 is a highly complex protein, it is difficult to manufacture in microbial expression systems.
TGF-β1 PLUS™ is the world’s first animal origin-free TGF-β1, manufactured for highly reproducible results.
Qkine TGF-β1 bioactivity
- TGF-β1 PLUS™ (Qk010) was bioactive in a quantitative luciferase assay with an EC50 of 34.8 pg/ml (lot #011) and 37 pg/ml (lot #012).
- This was more bioactive compared to mammalian expressed TGF-β1 from alternative suppliers, which had EC50s of 88.7 pg/ml (supplier 1) and 977 pg/ml (supplier 2).
Qkine TGF-β1 PLUS™ was found to be more bioactive than TGF- β1 from alternative suppliers. Bioactivity was determined using a TGF-β1-responsive (CAGA) firefly luciferase reporter in transiently transfected HEK293T cells. Cells were treated with a serial dilution of TGF-β1 PLUS™ (Qk010, green) or TGF-β1 from two alternative suppliers (black) for 6 hours in triplicate. Firefly luciferase activity was measured and normalized to the control, Renilla luciferase.
TGF-β1 PLUS™ shows exceptional lot-to-lot consistency, providing a reliable source of highly active TGF-β1. Qkine animal origin-free TGF-β1 PLUS was shown to be more bioactive than mammalian expressed alternatives. Qkine recombinant proteins are more cost effective, highly pure and animal origin-free, giving exceptional lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells.
Download PDFAll our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.