Technote | IL-6 (Qk093)

Qkine Interleukin-6 is more biologically active than a comparable alternative supplier protein

Interleukin 6 is a multifunctional cytokine that regulates immune responses and inflammation. It is produced by various cells, including immune cells such as T cells and macrophages, as well as non-immune cells like fibroblasts and endothelial cells. IL-6 is used for the culture of hematopoietic stem cells (HSC) and other relevant cells.

Qkine IL-6 (Qk093) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.

Qkine IL-6 (Qk093) bioactivity

  • Qkine Interleukin 6 (Qk093) was bioactive in a quantitative luciferase assay with EC50 of 1.85 ng/ml (88.5 pM).
  • This was more bioactive than Interleukin 6 from an alternative supplier, which had an EC50 of 5.74 ng/ml (274.6 pM).
Qkine IL-6 bioassay comparison

Quantitative luciferase reporter assay shows equivalent bioactivity of IL-6 (Qk093, green) and alternative supplier IL-6 (Supplier B black).
HEK293T luciferase reporter cells were treated in triplicate with a serial dilution of IL-6 for 24 hours. Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.

Qkine IL-6 (Qk093) was found to be more bioactive than IL-6 produced by an alternative major supplier. Qkine IL-6 (Qk093) has the advantage of being more cost effective, highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of HSC and other cells.

All our recombinant proteins are animal-free
& come with Bioactivity. Guaranteed.

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Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.

Contact our stem cell experts

Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.