Technote | GM-CSF (Qk076)
Qkine granulocyte macrophage colony-stimulating factor (GM-CSF) is as biologically active as the comparable alternative supplier protein
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor involved in differentiating and activating monocytes and granulocytes. It is also involved in developing erythroid cells, megakaryocytes, and keratinocytes. GM-CSF is commonly used in cell culture to stimulate the differentiation and maturation of human induced pluripotent stem cells or peripheral blood cells to myeloid lineages.
Qkine GM-CSF (Qk076) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine GM-CSF bioactivity
- Qkine GM-CSF (Qk076) stimulated proliferation of TF-1 human myeloid leukemia cells with an EC50 of 15.4 ng/ml (1 nM).
- This was comparable to Supplier A bacterially expressed GM-CSF bioactivity of 12.9 ng/ml (0.9 nM).
Qkine GM-CSF was found to be as bioactive as GM-CSF from an alternative major supplier. Bioactivity was determined using proliferation of TF-1 cells. Cells were treated in triplicate with a serial dilution of Qkine GM-CSF (Qk076, green) or Peprotech (300-03, Supplier B, black) for 48 hours. Protein concentrations were measured before use (Peprotech recovery 116%, Qkine 104%). Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.
The bioactivity comparison demonstrates that Qkine GM-CSF (Qk076) has equivalent bioactivity to GM-CSF from an alternative major supplier. Qkine GM-CSF has the advantage of being highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of myeloid cells and other relevant hematopoietic cells.
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