Technote | FGF-9 (Qk039)
Qkine Fibroblast Growth Factor 9 (FGF-9) bioactivity is comparable to an alternative supplier protein
Human FGF-9 protein is a member of the fibroblast growth factor (FGF) family, FGF-9 is involved in many developmental processes including neural and skeletal development. Recombinant FGF-9 is frequently used in the generation of kidney organoids.
Qkine FGF-9 (Qk039) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine FGF-9 bioactivity
- FGF-9 (Qk039) was bioactive in a quantitative luciferase assay with an EC50 of 21 ng/ml.
- This was comparable to FGF-9 from an alternative supplier, which had an EC50 of 22.6 ng/ml.
Qkine FGF-9 was as bioactive as FGF-9 from an alternative major supplier. Bioactivity was determined using the Promega serum response element (SRE) luciferase reporter assay in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine FGF9 (Qk039, green) or Peprotech FGF-9 (100-23-25UG, Supplier B, black) for 3 hours. Protein concentrations were measured before use (Peprotech vial recovery 63%, Qkine 111%). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. Data from Qk039 lot #204610.
Qkine recombinant proteins are highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
