Technote | FGF-18 (Qk069)

Qkine human Fibroblast Growth Factor-18 is as biologically active as a comparable alternative supplier protein

Fibroblast Growth Factor 18 (FGF-18), a member of the FGF family, characterized by its heparin-binding properties plays a significant role in regulating diverse biological processes such as embryonic development, skeletal and bone development, cartilage maintenance, angiogenesis and tissue repair.

FGF-18 is widely used to support cell culture maintenance and proliferation, promote chondrogenic and osteogenic differentiation of stem cells and stimulate angiogenesis.

Qkine FGF-18 (Qk069) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.

Qkine FGF-18 (Qk069) bioactivity

  • Qkine FGF-18 (Qk069) was bioactive in a quantitative luciferase assay with EC50 of 8.76 ng/ml (0.43 nM).
  • This was comparable to FGF-18 from an alternative supplier, which also had an EC50 of 9.45 pg/ml (0.47 nM).
Qkine FGF18 bioassay comparison

Quantitative luciferase reporter assay shows equivalent bioactivity of Qkine FGF-18 (Qk069, green) and alternative supplier FGF-18 (Supplier B black). HEK293T reporter cells were treated in triplicate with a serial dilution of FGF-18 for 3 hours. Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.

The bioactivity comparison demonstrates that Qkine FGF-18 (Qk069) has equivalent bioactivity to EGF from an alternative major supplier. Qkine FGF-18 (Qk069) has the advantage of being highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture and differentiation of stem cells.

All our recombinant proteins are animal-free
& come with Bioactivity. Guaranteed.

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Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.

Contact our stem cell experts

Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.