Technote | FGF-10 (Qk003)

Qkine Fibroblast Growth Factor 10 (FGF-10) is as biologically active as a comparable alternative supplier protein

Fibroblast growth factor 10 (FGF10) is involved in several different embryo and adult cell and tissue types, including mesenchymal, neuronal and epithelial cells. FGF-10 is used widely for stimulating the differentiation of induced pluripotent stem cells (iPSC) and embryonic stem cells (ESC) and promoting organoid formation.

Qkine FGF-10 (Qk003) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.

Qkine FGF-10 (Qk003) Bioactivity

  • Qkine FGF-10 (Qk003) was bioactive in a quantitative luciferase assay with EC50 of 1.32 ng/ml (77.6 pM).
  • This was comparable to FGF-10 from an alternative supplier, which had an EC50 of 1.48 ng/ml (87 pM).
Qkine Fibroblast growth factor 10 competitor comparison bioassay

Quantitative luciferase reporter assay shows equivalent bioactivity of Qkine FGF-10 (Qk003), green) and alternative supplier FGF-10 (Supplier B black). MCF-7 luciferase reporter cells were treated in triplicate with a serial dilution of FGF-10 for 4 hours. Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.

This bioactivity comparison demonstrates that Qkine FGF-10 (Qk003) has equivalent bioactivity to EGF from an alternative major supplier. Qkine FGF-10 (Qk003) has the advantage of being highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of stem and organoids.

All our recombinant proteins are animal-free
& come with Bioactivity. Guaranteed.

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Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.

Contact our stem cell experts

Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.