Technote | BMP-2 (Qk007)
Qkine bone morphogenetic protein 2 (BMP-2) has higher bioactivity than BMP-2 from an alternative supplier
Human bone morphogenetic protein 2 (BMP-2) is a key regulator of embryogenesis and supports the differentiation of induced pluripotent stem cells (iPSC) and embryonic stem cells (ECS). BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.
Qkine BMP-2 (Qk007) has high bioactivity, allowing lower concentrations to be used in media for cost-effective cell culture.
Qkine BMP-2 bioactivity
- Qkine animal origin-free BMP-2 (Qk007) was found to be highly bioactive with an EC50 of 3.2 ng/ml.
- This was higher than the bioactivity of an alternative supplier protein, which had an EC50 of 9.6 ng/ml.
Qkine BMP-2 has higher bioactivity than an alternative supplier. Bioactivity was determined using a BMP-2-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of Qkine BMP-2 (Qk007, green) or Peprotech (AF-120-02, supplier B, black). Protein concentrations were measured before use (Peprotech vial recovery 74%, Qkine 95%). Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk007 lot #204510.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.