Reconstitution solution A (Qk1001)
Solution of 10 mM HCl for use in the reconstitution of lyophilised proteins. For some proteins, physiological buffers may cause precipitation of stock solutions. Hence, we recommend dissolving our lyophilized TGF beta family and other related growth factors in 10 mM HCl.
Making life easy and keeping proteins happy
As protein experts, we are keen to make it as easy as possible for you to keep our lovely proteins in top condition. To this end, we are supplying 10 mM HCl with lyophilized proteins, for which this is our preferred reconstitution buffer. No needs to make buffers, and optimal protein performance – win win!
Reconstitution solution A is provided with lyophilized proteins and is free of charge.
For any questions, please contact us.
All our recombinant proteins are animal-free
and come with Bioactivity. Guaranteed.
Reconstitution instructions
1 – Check the resuspension solution recommendation on the product datasheet or use the quick reconstitution solution finder above*.
2 – Centrifuge briefly before reconstituting to ensure all powder is at the bottom of the vial.
3 – Add sufficient reconstitution solution to give a protein concentration of 0.05–1.0 mg/ml.
4 – Allow the reconstituted sample to stand at room temperature for >1 minute before gently agitating, do not vortex and avoid foaming.
5 – Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -80 °C (or -20 °C)**.
NOTES
*Physiological buffers may cause precipitation of proteins during reconstitution. This is especially important for TGF beta family and other related growth factors so it is very important that these are reconstituted in Solution A (10 mM HCl, 1:1000 dilution of concentrated HCl).
**All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 μg/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
Reconstitution calculator
Use our reconstitution calculator to determine the necessary volume of a reagent for reconstituting your vial.
Enter the mass of your protein and the desired concentration of your final solution, then click solve.
Desired concentration (μg/ml)
