The bioactivity of Qk076 GM-CSF and bacterial-expressed GM-CSF from an alternative supplier is determined using the proliferation of TF-1 human myeloid leukemia cells. Cells are treated in triplicate with a serial dilution of GM-CSF for 48 hours. Cell viability is measured using the CellTiter-Glo (Promega) luminescence assay and normalized.
The bioactivity comparison demonstrates that Qkine GM-CSF (Qk076) has equivalent bioactivity to GM-CSF from an alternative major supplier. Qkine granulocyte macrophage colony-stimulating factor provides a reliable source of highly pure animal-free GM-CSF for the reproducible culture of myeloid and other relevant hematopoietic cells.
Qkine GM-CSF, along with all Qkine recombinant proteins, come with a Bioactivity Guarantee.