Technote | GM-CSF (Qk076)
Qkine granulocyte macrophage colony-stimulating factor (GM-CSF) is stable and bioactive up to 28 days in cell culture conditions
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor involved in differentiating and activating monocytes and granulocytes. It is also involved in developing erythroid cells, megakaryocytes, and keratinocytes. GM-CSF is commonly used in cell culture to stimulate the differentiation and maturation of human induced pluripotent stem cells or peripheral blood cells to myeloid lineages.
Qkine GM-CSF (Qk076) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine GM-CSF stability
- After 14 days Qkine GM-CSF (Qk076) stimulated proliferation of TF-1 human myeloid leukemia cells with the same potency as fresh GM-CSF, with an EC50 at day 0 of 68 pg/ml and 80 pg/ml at day 14.
- GM-CSF was still bioactive after 28 days, with an EC50 at day 0 of 71 pg/ml and 73 pg/ml at day 28.
- No degradation of protein can be seen in an SDS-PAGE gel after 14 or 28 days.
Bioactivity and stability of Qkine GM-CSF. Bioactivity was determined using proliferation of TF-1 human myeloid leukaemia cells. Cells were treated in triplicate for 48 hours with a serial dilution of GM-CSF which had been pre-incubated in conditioned media at 37°C for (A) 14 days or (B) 28 days. Cell viability was measured using CellTiter-Glo (Promega) luminescence assay. (C) SDS-PAGE of recombinant GM-CSF showed that protein was not degraded when incubated in HEK293T conditioned media for 14 or 28 days at 37°C.
Qkine GM-CSF (Qk076) is stable and bioactive under cell culture conditions for up to 28 days. Qkine GM-CSF is highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of myeloid cells and other relevant hematopoietic cells.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.