Currency
Recombinant human vitronectin protein (Qk120)
Vitronectin protein is widely used in stem cell culture. It provides a defined environment that supports the maintenance of pluripotency and is suitable for feeder-free culture, expansion, differentiation, and reprogramming of stem cells.
Qkine vitronectin is a high-purity animal origin-free recombinant protein with a molecular weight of 47.8 kDa. It is carrier-free and protein tag-free, ensuring exceptional lot-to-lot consistency and compatibility with translational studies and iPSC-based model production for screening. Qkine recombinant vitronectin protein is ultra high-purity with exceptionally low residual endotoxins making it suitable for the reproducible culture of stem cells, primary cells, organoids, and use in sensitive neural differentiations.
Our robust animal origin-free manufacturing platform, along with rigorous quality control procedures, ensures exceptional bioactivity and consistent performance from lot to lot, guaranteeing outstanding performance in your applications.
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
5000µg (5mg) will be despatched as 10x 500µg
Fast and free shipping.
Buy online with secure credit card or purchase order.
Summary
Highly pure recombinant human vitronectin protein (truncated) (UniProt number: P04004)
>95%, by SDS-PAGE quantitative densitometry
Source: Expressed in E. coli
- 47.8 kDa, monomer
- Animal origin-free (AOF) and carrier protein-free
Manufactured in Cambridge, UK
- Lyophilized from PBS, mannitol and TCEP
Resuspend in sterile-filtered water at 1 mg/m, add carrier protein if desired, and store frozen at -20°C or -80°C
Featured applications
Maintenance and expansion of human iPSC, ESC and primary cells
Directed differentiation of iPSCs towards multiple lineages (tri lineage differentiation protocols)
- Promotion of cell adhesion and migration
Generation of tissue-specific organoids
- Stimulation of angiogenesis and vascular network development
Fully defined, animal-free protocols for translational studies and preclinical stem cell production
Truncated Vitronectin
VTN-N
S-protein
Serum-spreading factor, V75
human
species similarity:
mouse – 74%
rat – 76%
porcine – 74%
bovine – 72%
Frequently used together
Recombinant human FGF-2 (145 aa) protein (Qk025)
Recombinant human FGF-2 (154 aa) protein (Qk027)
Recombinant FGF2-G3 (145 aa) protein (Qk052)
Recombinant FGF2-G3 (154 aa) protein (Qk053)
Recombinant human TGF-ß1 PLUS protein (Qk010)
Recombinant human TGF-β3 protein (Qk054)
Recombinant human VEGF 165 protein (Qk048)
Recombinant human PDGF-BB protein (Qk044)
Recombinant human IGF-1 protein (Qk047)
Recombinant human PDGF-AA protein (Qk043)
Imaging of colonies grown on Qkine vitronectin coated plates in E8-like media retained their highly preserved morphological appearance. (A) after initial seeding and 3 days in culture; (B) After 1 passages and 7 days in culture; (C) After 3 passages and 14 days in culture (scale bar = 150µm). 6-well plates were coated with 5 µg/ml Qk120 vitronectin protein lot #204660.
Further quality assays
Mass spectrometry, single species with the expected mass
Endotoxin: <0.005 EU/μg protein (below the level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Preparation of cell culture plates with Qkine ultra-high quality vitronectin (Qk120)
- Briefly centrifuge vial containing vitronectin Qk120 to ensure all lyophilized protein is collected at the bottom of the vial.
- Resuspend in 500 ml of MilliQ water to make a 1 mg/ml stock and lightly agitate the tube to ensure everything has fully reconstituted.
- Per 6-well plate required, dilute 30 µl of the 1 mg/ml in 6 ml of phosphate buffered saline (PBS) without MgCl and CaCl to make 5 µg/ml solution.
- Coat each well of 6-well plate with 1 ml per well of 5 µg/ml vitronectin for at least two hours at 37°C.
- Adjust volume of 5 µg/ml vitronectin per well for different area plate clusters.
- 12-well plate add 500 µl
- 24-well plate add 250 µl
- 96-well plate add 100 µl
- Aliquot the remaining resuspended 1 mg/ml vitronectin into appropriately sized single use aliquots and store at -80°C.
Protein background
In tissue engineering and regenerative medicine, vitronectin-coated scaffolds and biomaterials enhance cell attachment, proliferation, and differentiation, contributing to the development of functional tissue constructs [15-16]. Additionally, vitronectin is used to prepare cells for cell therapy, ensuring their viability and functionality upon transplantation.
Vitronectin is particularly important for optimizing culture conditions in the manufacturing of serum-free media, compensating for the lack of adhesion-promoting factors typically provided by serum and supporting cell growth under defined conditions [7, 17]. The use of recombinant vitronectin protein ensures batch-to-batch consistency, which is critical for reproducibility in experimental and clinical applications.
Structurally, vitronectin is a multifunctional glycoprotein with a molecular weight of approximately 75 kDa. It comprises several domains, including the somatomedin B domain at the N-terminal, which is important for binding to the urokinase receptor and stabilizing the inactive form of plasminogen activator inhibitor-1 (PAI-1). The RCL (Ricinus communis agglutinin-like) domain is crucial for heparin binding, while the hemopexin-like repeats facilitate interactions with integrins and other ECM components [2,6,18]. Vitronectin can undergo conformational changes that expose or hide specific binding sites, enabling it to interact with a wide range of ligands and receptors.
In summary, Vitronectin plays a crucial role in various applications, particularly in stem cell and organoid applications, by providing a defined and supportive environment for stem cell maintenance, differentiation, and reprogramming. Its use enhances the reproducibility and efficiency of several cell cultures, making it a valuable tool in both research and therapeutic contexts.
Additional resources
Weekend-free human induced pluripotent stem cell culture using thermostable FGF-2 (bFGF) and animal origin-free TGF-β1 and vitronectin for improved colony homogeneity
FAQ
A glycoprotein found in the extracellular matrix (ECM) that plays critical roles in cell adhesion, migration, wound healing and regulation of immune response.
Vitronectin is found in the extracellular matrix, blood plasma and various tissues.
Vitronectin is not a cytokine but it signals through integrin receptors to support maintenance of pluripotency during iPSC culture.
Vitronectin gene encodes vitronectin protein, which is a component of the extracellular matrix.
Vitronectin binds to integrins, heparin and heparan sulfate proteoglycans, plasminogen activator inhibitor – 1, complement system.
The vitronectin receptor facilitates cell adhesion, cell migration and initiates signaling processes that promotes cell survival, proliferation and differentiation.
Qkine products are intended solely for research purposes and ex vivo cell manufacturing. Not for therapeutic or diagnostic use.
What others are saying
There are no contributions yet.