Recombinant human TPO protein (Qk098-CTG) Price range: £3,795.00 through £5,940.00View product
Recombinant human TPO protein (Qk098-CTG)
Price range: £3,795.00 through £5,940.00
Thrombopoietin (TPO) is a cytokine which stimulates the production and differentiation of megakaryocytes, regulating platelet production. Recombinant TPO can be used to support the self-renewal and maintenance of hematopoietic stem cells.
Qkine TPO is a 16 kDa protein, animal origin-free, tag-free and carrier-free it has been manufactured to be highly pure and bioactive for reproducible hematopoietic stem cell culture.
Qkine cell therapy grade high purity animal origin-free proteins are manufactured as GMP grade equivalents in an ISO 9001:2015-certified facility, under ISO 20399:2022 standards with GMP compliance, defined quality criteria and documentation.Â
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Orders are typically shipped same or next day (except Friday).
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Price range: £3,795.00 through £5,940.00
1000µg will be despatched as 2 x 500µg
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Summary:
- High purity human protein (Uniprot: P07202)
- 16 kDa (monomer)
Certified animal origin-free (AOF)
Cell and gene therapy grade
Manufactured in our ISO 9001:2015 facility in Cambridge, UK
Lot-to-lot consistency in bioactivity
Full traceability and documentation
Carrier protein-free, tag free
Expressed in E. coli
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Immune cell therapy expansion
Alternative proteins
Recombinant TPO activity was determined using a Promega serum response element luciferase reporter assay in HEK293T cells co-transfected with TPOR. Cells were treated in triplicate with a serial dilution of TPO for 3 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk098 lot 204752. EC50 = 4.72 ng/ml (0.30 pM).
Recombinant TPO migrates at approximately 14 kDa (monomer) in reduced (R) and non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk098 lot #204752.
Further quality assays
Mass spectrometry: single species with expected mass
Purity >98%, by SDS-PAGE quantitative densitometry
Recovery from stock vial:Â >95%
Sterility tested and mycoplasma negative
Endotoxin: <0.005 EU/μg protein (below level of detection)
Residual host cell protein <10 ng/µg
Residual host cell DNA <10 ng/μg
N-terminal sequence analysisÂ
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
TPO is a class I hematopoietic cytokine encoded by a gene located on chromosome 3q26-27. The TPO protein consists of 355 amino acids with the first 155 being homologous to erythropoietin (EPO) [1]. TPO binds to its receptor, Mpl and regulates proliferation and maturation of megakaryocytes as well as platelet production [1, 2].
TPO is primarily synthesized in the liver but can also be produced by the kidney, spleen, and bone marrow. In the liver, its production is constitutive and not linked to anemia like its homolog EPO [2]. Reduced platelet production leads to thrombocytopenia, a common hematological disorder which can lead to spontaneous bleeding, frequently as a side effect of cancer treatment [4, 5]. Severe cases may require a platelet transfusion [4]. However, the use of recombinant TPO as a treatment for thrombocytopenia has been investigated and has shown to increase platelet count [5].
TPO can also be used in the culture and differentiation of hematopoietic stem cells (HSCs) for research and therapeutic applications. TPO can support ex vivo expansion of HSC in combination with Flt3L and SCF [2]. In addition, it also increases the number of erythroid and myeloid progenitors, in synergy with stem cell factor (SCF) and interleukin-3 (IL-3) [3].
The TPO receptor Mpl is expressed primarily on megakaryocytes and platelets. TPO binding to Mpl causes receptor dimerization and phosphorylation by JAK2. This activates multiple downstream signaling pathways including STATs, the MAPK pathway, and the PI3K pathway [2].
Additional resources
FAQ
How is TPO used in cell culture?
TPO can be used in cell culture in the differentiation of hematopoietic stem cells (HSCs) and maturation of megakaryocytes.
Is TPO a cytokine?
Yes, Thrombopoietin (TPO) is a cytokine and is also classed as a hormone.
What does TPO bind to?
TPO binds to the myeloproliferative leukemia protein (MPL) or Cluster of Differentiation 110 (CD110), also known as the thrombopoietin receptor (TPO-R).
What is the function of the TPO receptor?
The TPO receptor MPL plays a crucial role in hematopoiesis, particularly in the development of megakaryocytes and platelets.
What is the TPO pathway?
Binding of TPO to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis).
What is TPO?
Thrombopoietin or TPO is a cytokine produced by the liver and kidney and regulates the production of platelets.
Where is TPO found?
TPO is primarily produced in the liver and kidney and is found in circulating plasma.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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