Recombinant human IL-7 protein (Qk095-CTG) Price range: £4,550.00 through £6,880.00View product
Recombinant human IL-7 protein (Qk095-CTG)
Price range: £4,550.00 through £6,880.00
Interleukin-7 (IL-7) is a vital cytokine essential for immune system regulation, particularly in the development and maintenance of T cells, playing a crucial role in both adaptive and innate immune responses. Recombinant human IL-7 stimulates the development of lymphoid progenitor cells.
Qkine has optimized the IL-7 manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility. Cell therapy grade IL-7 is a highly pure and bioactive 17.5 kDa monomer with extensive quality testing.
Qkine cell therapy grade high purity animal origin-free proteins are manufactured as GMP grade equivalents in an ISO 9001:2015-certified facility, under ISO 20399:2022 standards with GMP compliance, defined quality criteria and documentation.Â
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Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £4,550.00 through £6,880.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human IL-7 protein (Uniprot number: P13232)
- 17.5 kDa (monomer)
Certified animal origin-free (AOF)
Cell and gene therapy grade
Manufactured in our ISO 9001:2015 facility in Cambridge, UK
Lot-to-lot consistency in bioactivity
Full traceability and documentation
Carrier protein-free, tag free
Expressed in E. coli
Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Immune cell therapy expansion
CAR-T cell therapy manufacturing
Alternative proteins
Recombinant IL-7 activity was determined using proliferation of mouse-derived B lymphocyte cell line 2E8. Cells were treated in duplicate with a serial dilution of IL-7 for 65 hours. Cell viability was measured using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (Promega). Data from Qk095 lot 204669. EC50 = 0.40 ng/ml (23 pM).
Recombinant IL-7 migrates as a major band at approximately 17.5 kDa (monomer) in reduced (R) and non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk095 lot #204669.
Further quality assays
Mass spectrometry: single species with expected mass
Purity >98%, by SDS-PAGE quantitative densitometry
Recovery from stock vial:Â >95%
Sterility tested and mycoplasma negative
Endotoxin: <0.005 EU/μg protein (below level of detection)
Residual host cell protein <10 ng/µg
Residual host cell DNA <10 ng/μg
N-terminal sequence analysisÂ
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine IL-7 was mores bioactive than the WHO (NIBSC) standard IL-7. IL-7 activity was determined using a SRE luciferase reporter assay in transiently transfected HEK293 cells co-transfected with IL-7Rα and IL-7Rγ. Transfected cells were treated in triplicate with a serial dilution of IL-7 for 3 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Qk095 204669 EC50 = 1.20 ng/ml (69 pM). WHO (NIBSC) standard EC50 = 3.40 ng/ml.
Protein background
IL-7 is a non-glycosylated protein with a molecular weight of about 17.5 kDa. It features four alpha-helices that facilitate its interaction with the IL-7 receptor (IL-7R), a heterodimer consisting of the IL-7Rα chain (CD127) and the common gamma chain (γc, CD132). This binding initiates signaling through the JAK-STAT pathway, particularly involving STAT5 phosphorylation, which promotes T cell survival, proliferation, and differentiation [1].
IL-7 is essential for the survival and homeostasis of T cells, supporting the development of thymocytes in the thymus and maintaining naive and memory T cells in the periphery, thus ensuring effective immune surveillance and long-term memory [2]. IL-7 influences B cell development and plays a role, though less prominently, in the development of natural killer (NK) cells [2].
IL-7 is extensively studied for its potential in cancer immunotherapy, where it enhances immune responses, particularly in promoting T cell recovery following chemotherapy or radiation, and in combination with checkpoint inhibitors [4]. In HIV and other chronic infections, IL-7 is explored for its ability to restore immune function by increasing CD4+ T cell counts and reducing immune exhaustion, potentially improving the effectiveness of existing therapies [3]. IL-7 also plays a key role in bone marrow transplantation, where it accelerates T cell recovery, reduces immune vulnerability, and is studied for its potential to mitigate graft-versus-host disease (GVHD) [4, 5].
In autoimmune diseases like multiple sclerosis and rheumatoid arthritis, IL-7 is a target for controlling autoreactive T cell survival and proliferation, offering new therapeutic approaches [5]. IL-7 is investigated as a vaccine adjuvant, particularly in vaccines requiring strong T cell responses, and in regenerative medicine, where its role in expanding T cells and hematopoietic stem cells is harnessed to enhance the efficacy of immune system regeneration therapies [4-6].
Additional resources
FAQ
What does IL-7 bind to?
IL-7 binds to the IL-7 receptor, composed of the IL-7Rα (CD127) and the common gamma chain (γc, CD132).
How is IL-7 used in cell culture?
IL-7 is used to support the growth, survival, and proliferation of T cells and B cell precursors. It’s particularly vital in expanding T cells for research, immunotherapy, and studying immune cell development in vitro.
Is IL-7 a cytokine?
Yes, IL-7 is a cytokine. Cytokines are signaling molecules that mediate and regulate immunity, inflammation, and hematopoiesis.
What does the IL-7 gene do?
The IL-7 gene encodes the protein interleukin-7 (IL-7), which is a cytokine critical for the development and maintenance of the immune system, particularly in T cells.
What is IL-7?
IL-7 is an essential cytokine involved in the development, survival, and maintenance of T-cells, crucial for the immune response. It supports T-cell homeostasis, aids in B-cell development, and is being studied for therapeutic potential in immunodeficiency disorders, cancer treatment, and enhancing vaccine responses by boosting immune function.
What is the function of the IL-7 receptor?
The IL-7 receptor (IL-7R) functions to mediate the survival, proliferation, and differentiation of T cells. It does this by binding IL-7 and triggering signaling pathways, particularly the JAK-STAT pathway, which promotes T cell development in the thymus, maintains peripheral T cell homeostasis, and supports early B cell development.
What is the IL-7 signaling pathway?
The IL-7 pathway involves IL-7 binding to its receptor, activating the JAK-STAT signaling cascade, which promotes T cell survival, proliferation, and differentiation.
Where is IL-7 found?
IL-7 is primarily produced by stromal cells in the bone marrow and thymus. It is also found in secondary lymphoid organs, such as lymph nodes, and is produced by non-lymphoid tissues like the skin, gut, and liver.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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