Recombinant human IL-15 protein (Qk097-CTG) Price range: £3,795.00 through £5,940.00View product
Recombinant human IL-15 protein (Qk097-CTG)
Price range: £3,795.00 through £5,940.00
Interleukin-15 (IL-15) is a pro-inflammatory cytokine which regulates immune responses. Recombinant IL-15 promotes the growth and activation of T-cells and B cells and is essential in the development, survival and activation of NK cells. IL-15 can also promote conversion of monocytes into dendritic cells.
Qkine has optimized the IL-15 manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility. Qkine cell therapy grade IL-15 is a highly pure 12.9 kDa monomer, animal origin-free (AOF) and carrier-protein-free (CF).
Qkine cell therapy grade high purity animal origin-free proteins are manufactured as GMP grade equivalents in an ISO 9001:2015-certified facility, under ISO 20399:2022 standards with GMP compliance, defined quality criteria and documentation.Â
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £3,795.00 through £5,940.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human IL-15 protein (Uniprot number: P40933)
- 12.9 kDa (monomer)
Certified animal origin-free (AOF)
Cell and gene therapy grade
Manufactured in our ISO 9001:2015 facility in Cambridge, UK
Lot-to-lot consistency in bioactivity
Full traceability and documentation
Carrier protein-free, tag free
Expressed in E. coli
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Immune cell therapy expansion
CAR-T cell therapy manufacturing
Alternative proteins
Recombinant IL-15 activity was determined using a SRE luciferase reporter assay in transiently transfected HEK293 cells co-transfected with the IL-15 receptors (IL-2Rβ and IL-2Rγ). Transfected cells were treated in triplicate with a serial dilution of IL-15 for 3 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity.
The specific activity of recombinant human IL-15 GMP is >1.5 x 107 units/mg, which is calibrated against the human IL-15 standard (NIBSC code: 95/554).
Recombinant IL-15 migrates as a band at approximately 12 kDa (monomer) in reduced (R) and 9 kDa in non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein 3 µg was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk097 lot #204728.
Further quality assays
Mass spectrometry: single species with expected mass
Purity >98%, by SDS-PAGE quantitative densitometry
Recovery from stock vial:Â >95%
Sterility tested and mycoplasma negative
Endotoxin: <0.005 EU/μg protein (below level of detection)
Residual host cell protein <10 ng/µg
Residual host cell DNA <10 ng/μg
N-terminal sequence analysisÂ
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine IL-15 was more bioactive than the WHO (NIBSC) standard IL-15. IL-15 activity was determined using a SRE luciferase reporter assay in transiently transfected HEK293 cells co-transfected with the IL-15 receptors (IL-2Rβ and IL-2Rγ). Transfected cells were treated in triplicate with a serial dilution of IL-15 for 3 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Qk095 #204736 EC50 = 0.25 ng/ml (19.4 pM). WHO (NIBSC) standard EC50 = 0.57 ng/ml.Â
Protein background
Interleukin-15 (IL-15) is a pro-inflammatory cytokine expressed in a large variety of tissues and cell types including epithelial cells, fibroblasts, keratinocytes, nerve cells, monocytes, macrophages, and dendritic cells [1]. IL-15 shares many biological properties with IL-2, despite not having sequence homology, as it interacts with components of the IL-2 receptor (IL-2R) [1, 2]. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6 [2].
Recombinant IL-15 stimulates the proliferation and activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells [3]. IL-15 is also essential for NK cell development and can be used for the development of NK cells from hematopoietic progenitor cells [4,5].
IL-15 has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes. IL-15 has also been implicated in both the development and treatment of cancers [1]. Recombinant IL-15 can also be used for the preconditioning of CAR T cells or for engineering cells to express IL-15 in vivo [6].
Additional resources
FAQ
Is IL-15 a cytokine?
Yes, Interleukin-15 (IL-15) is a pleiotropic cytokine belonging to the common gamma-chain (γc) cytokine family, which also includes IL-2, IL-4, IL-7, IL-9, and IL-21.
What cells express the IL-15 receptor?
The IL-15 receptor consists of the IL-2Rβ and γc subunits in association with a unique ligand-specific subunit, IL-15Rα, IL-15Rα is expressed by lymphoid cells, DC, fibroblasts, epithelial, liver, intestine, and other cells.
What cells respond to IL-15?
IL-15 binds to and activates CD8+ T cells, NKT cells, γδT cells, and NK cells.
What cells secrete IL-15?
IL-15 is secreted primarily by dendritic cells, monocytes, and epithelial cells.
What does the IL-15 gene do?
The IL15 gene is localized on chromosome 4q31 and codes for interleukin-15 (IL-15), a widely expressed pro-inflammatory cytokine.
What is IL-15?
IL-15 is an inflammatory cytokine.
What is the IL-15 signaling pathway?
IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6.
What is the function of the IL-15 receptor?
IL-15RA is expressed on the surface of activated and memory CD8+ T cells, it plays an important role in innate and adaptive immunity.
Where is IL-15 found?
IL-15 is a soluble protein found in blood and tissues.
How is recombinant IL-15 used in cell culture?
IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It can also be used to develop monocytes into dendritic cells.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
Receive an Amazon gift voucher when you leave us a review.
£25, $30 or €30 for reviews with an image and £10, $15 or €15 for reviews without an image