Recombinant human EPO protein (Qk099) Price range: £140.00 through £1,400.00View product
Recombinant human EPO protein (Qk099)
Price range: £140.00 through £1,400.00
Erythropoietin (EPO) is an essential cytokine hormone which controls the production of erythrocytes (red blood cells). Recombinant EPO can be used in the differentiation of hematopoietic projector cells into erythrocytes and other major hematopoietic lineages.
Qkine have developed the only commercially available bioactive animal origin-free EPO for reproducible hematopoietic stem cell culture. Qkine EPO is a 18.4 kDa protein, tag-free and carrier-free.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £140.00 through £1,400.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human protein (Uniprot: P01588)
- 18.4 kDa (monomer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Maintenance and differentiation of hematopoietic stem cells
Proliferation and differentiation of erythrocytes, leukocytes and plateletsÂ
Maintenance of cardiac progenitor cells
Recombinant EPO activity was determined using proliferation of TF-1 human myeloid leukemia cells. Cells were treated in triplicate with a serial dilution of EPO for 72 hours. Cell viability was measured using the CellTiter-Glo (Promega) luminescence assay. Data from Qk099 lot #204757. EC50 = 1.14 ng/ml (62 pM).
Recombinant EPO migrates as a major band at approximately 18 kDa (monomer) in reduced (R) and 16 kDa (monomer) in non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk099 lot #204757.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Erythropoietin (EPO) is an essential cytokine which controls erythropoiesis, the production of red blood cells. Mainly produced by the kidneys, human EPO is a 30.4 kDa glycoprotein hormone [1]. Basal levels of EPO are essential for maintaining circulating red blood cell and hemoglobin levels, loss of EPO due to development of neutralizing antibodies or chronic kidney disease leads to anemia [1,2]. Increased expression of EPO is stimulated by hypoxia promoting the viability, proliferation, and terminal differentiation of erythroid precursors, resulting in an increase in red blood cell mass [3].
EPO binds to the EPO receptor (EPOR), a member of the cytokine receptor superfamily which consists of a single membrane-spanning domain, receptor dimerization upon ligand binding leads to phosphorylation and activation by Janus kinase 2 (JAK-2) [1]. This activates downstream signaling pathways including STAT-5, phosphatidyl-inositol 3-kinase (PI-3K)/AKT (protein kinase B), and SHC/mitogen-activated protein kinase (MAPK) [1, 2].
Recombinant EPO, in combination with SCF, TPO, Flt3L, IL-7 and IL-15, can be used to differentiate hematopoietic progenitors into eight hematopoietic lineages (erythrocytes, platelets, macrophages, granulocytes, dendritic cells, natural killer cells, B cells, and T cells) [4]. EPO is also used in the culture of cardiac progenitor cells [5]. Studies show human EPO is suitable for the culture of mouse hematopoietic and cardiac progenitor cells [4, 5].
Additional resources
FAQ
What is EPO?
Erythropoietin (EPO) is a cytokine which is essential for red blood cell production.
Where is EPO found?
EPO is found in the blood stream, it is mostly produced in the kidneys and acts in the bone marrow, but has other tissue specific effects.
Is EPO a cytokine?
Yes, it is also a hormone
What does the EPO gene do?
The EPO gene encodes Erythropoietin, a secreted, glycosylated cytokine composed of four alpha helical bundles.
What does EPO bind to?
EPO binds to the EPO receptor, EPOR.
What is the function of the EPO receptor?
Binding of EPO to the EPO R activates downstream signaling pathways responsible for the viability, proliferation, and terminal differentiation of erythroid precursors, resulting in an increase in red blood cell production.
What is the EPO pathway?
Binding of EPO to the EPO R activates downstream signaling pathways including STAT5, PI3-kinase and MAPK.
How is EPO used in cell culture?
EPO can be added to cell culture media to promote the differentiation of hematopoietic progenitors. EPO can be used in the culture of cardiac progenitor cells.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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