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Qk115-FG bpNRG-1 bioactivity
High quality food grade for cultivated meat, fish, fat and dairy

Recombinant bovine/porcine NRG-1 protein (Qk115-FG)

Bovine/porcine NRG-1 protein (Neuregulin 1) is frequently used in the maintenance of pluripotent stem cells.  In addition to its widespread use in stem cell culture media, NRG-1 (also known as Heregulin-β1 , HRG-1) has essential roles in vivo including in nervous system, cardiac, and mammary gland development; cancer biology and neurological disorders.

Qkine high quality food grade NRG-1 is the 7.5 kDa bioactive domain of human NRG-1, comprised of the β isoform of the EGF-like domain of NRG-1 (HRG1-B1). This NRG-1 protein monomer is animal origin-free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg will be despatched as 2 x 500µg

Available on request.
For any questions, please email orders@qkine.com

Summary

  • High quality bovine/porcine NRG-1 protein (Uniprot: A3KN34)

  • >98%, by SDS-PAGE quantitative densitometry

  • 7.5 kDa

  • Expressed in E. coli. 

  • Animal origin-free (AOF) and carrier protein-free.

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from acetonitrile, TFA

  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Featured applications

  • High quality food grade recombinant protein

  • Cultivated meat and fat

Neuregulin1 (NRG1-β1), Neu differentiation factor (rat), Heregulin, HRG, HRG1-β1, Breast cancer cell differentiation factor p45, ARIA (Acetylcholine, Receptor Inducing Activity), glial growth factor

Human
Porcine (pig)
Bovine (cow)
Mouse (expected, 98.5% identity)

Related discovery kits

B8 media discovery kit (Qk503)
Beefy9 media discovery kit (Qk504)

Bioactivity

Human LIF Qk036 protein bioactivity lot #14293

NRG-1 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. Cells are treated in triplicate with a serial dilution of NRG-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 37.9 pM (0.28 ng/ml). *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

Human LIF Qk036 protein purity SDS-PAGE lot #14293

NRG-1 migrates as a single band at 7.5 kDa in non-reducing (NR) conditions and upon reduction (R).  No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Analytical reversed-phase: single sharp peak 

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial >95%

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine.  To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Neuregulin 1 (NRG-1) is a member of the neuregulin family (along with NRG 2-4). Each member of this family is encoded by an individual gene.  Alternative splicing and different promoter utilisation results in a large number of isoforms for each member of the protein family – for example, NRG-1, the best studied member, generates six types of protein (I–VI) and at least 31 isoforms [1]. These isoforms have different tissue distribution, potency, receptor specificity and biological functions [2].

All NRG-1 isoforms contain the bioactive EGF-like domain, this EGF-like domain has two isoforms: alpha and beta. These differ in their receptor affinity; both the alpha and beta isoforms are ligands for the receptors ErbB3 (HER3) and ErbB4 (HER4), however the NRG-1 beta isoform has a higher affinity for the ErbB4 receptor than the alpha isoform [3]. This protein (Qk115-FG) consists of the β isoform of the EGF domain of NRG-1 protein.

NRG-1 has an essential role in cardiac and neuronal development, and is implicated in disorders such as schizophrenia [4], and numerous cancers, for example breast cancer where NRG-1 promotes the proliferation and migration of breast cancer cells [5]. In cell culture, NRG-1 is frequently used in maintenance media for pluripotent stem cells and other cells.

[1] Mei, Lin, and Wen-Cheng Xiong. ‘Neuregulin 1 in Neural Development, Synaptic Plasticity and Schizophrenia’. Nature Reviews Neuroscience, vol. 9, no. 6, June 2008, pp. 437–52. doi.org/10.1038/nrn2392.[2] Breuleux, M. ‘Role of Heregulin in Human Cancer’. Cellular and Molecular Life Sciences, vol. 64, no. 18, Sept. 2007, pp. 2358–77. doi.org/10.1007/s00018-007-7120-0.[3] Hobbs, Stuart S., et al. ‘Neuregulin Isoforms Exhibit Distinct Patterns of ErbB Family Receptor Activation’. Oncogene, vol. 21, no. 55, Dec. 2002, pp. 8442–52. doi.org/10.1038/sj.onc.1205960.[4] Shi, Liang, and Clare M. Bergson. ‘Neuregulin 1: An Intriguing Therapeutic Target for Neurodevelopmental Disorders’. Translational Psychiatry, vol. 10, no. 1, June 2020, pp. 1–11. doi.org/10.1038/s41398-020-00868-5.[5] Berdiel-Acer, Mireia, et al. ‘Stromal NRG1 in Luminal Breast Cancer Defines Pro-Fibrotic and Migratory Cancer-Associated Fibroblasts’. Oncogene, vol. 40, no. 15, Apr. 2021, pp. 2651–66. doi.org/10.1038/s41388-021-01719-3.

Qkine high quality food grade products are intended solely for use as food processing aids, ex vivo cell manufacturing and research use. Not for direct human consumption, therapeutic or diagnostic use.

The authorization of novel foods, including the use of growth factors as food processing aids, is regulated by regional government agencies. The use of our products as food processing aids in novel foods requires the end-user to obtain the necessary regulatory approvals.

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