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Qk113-FG bpIGF-1 bioactivity
High quality food grade for cultivated meat, fish, fat and dairy

Recombinant bovine/porcine IGF-1 protein (Qk113-FG)

Bovine/porcine insulin-like growth factor 1 (IGF-1) protein is used in the maintenance of induced pluripotent stem cells (iPSC) and is necessary for cell growth in the absence of insulin. IGF-1 promotes the proliferation of many cell types, including embryonic stem cells (ESC) and mesenchymal stem cells (MSC).

Qkine bovine/porcine IGF-1 is a high quality food grade 8 kDa protein, Qkine growth factors are animal origin-free (AOF) and carrier-protein free (CF) for consistent stem cell culture and cultured meat applications.

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg will be despatched as 2 x 500µg

Fast and free shipping.
Buy online with secure credit card or purchase order.
For any questions, please email orders@qkine.com

Summary

  • High quality bovine/porcine IGF-1 protein (Uniprot: P07455)

  • >98%, by SDS-PAGE quantitative densitometry

  • 8 kDa

  • Expressed in E. coli. 

  • Animal origin-free (AOF) and carrier protein-free.

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from acetonitrile, TFA

  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Featured applications

  • High quality food grade recombinant protein

  • Cell culture media optimisation and replacement of insulin in defined media

Insulin-like Growth Factor-I, Somatamedin-C, Mechano Growth Factor (MGF)

human
porcine (pig)
bovine (cow)

Frequently used together

Recombinant bovine/porcine FGF2-G3 145 aa protein (Qk080-FG)

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B8 media discovery kit (Qk503)
Beefy9 media discovery kit (Qk504)

Bioactivity

Human LIF Qk036 protein bioactivity lot #14293

IGF-1 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM). Cells are treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk047 lot #104331. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

Human LIF Qk036 protein purity SDS-PAGE lot #14293

IGF-1 protein migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R).  No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk047 batch #104331.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Analytical reversed-phase: single sharp peak 

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial >95%

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine.  To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

The insulin-like growth factors (IGFs) are a family of proteins with a similar structure to insulin. IGF-1 has an A and B chain connected by disulphide bonds, its similarity to insulin means it can bind with low affinity to the insulin receptor [1]. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic, and lung [1]. IGF-1 exerts its biological effect through binding to the IGF1 receptor (IGF1R), its effect is modulated through interaction with a group of binding proteins (IGFBPs), which tend to inhibit the effect of IGF-1 [2]. Through IGF1R the Ras-MAPK and PI3 kinase signaling pathways are activated leading to increased cell proliferation and survival [2].

IGF-1 can be used to support maintenance of human embryonic stem cell (ESC) [3] and mesenchymal stem cell (MSC) cultures [3, 4].

[1] Z. Laron. Insulin-like growth factor 1 (IGF-1): a growth hormone. Mol Pathol. 2001 Oct;54(5):311-6. doi: 10.1136/mp.54.5.311

[2] H. Werner. The IGF1 Signaling Pathway: From Basic Concepts to Therapeutic Opportunities. International Journal of Molecular Sciences. 2023; 24(19):14882. doi.org/10.3390/ijms241914882

[3] L. Wang, T.C. Schulz, E. S. Sherrer, D. S. Dauphin, S. Shin, A. M. Nelson, C. B. Ware, M. Zhan, C. Z. Song, X. Chen, S. N. Brimble, A. McLean, M. J. Galeano, E. W. Uhl, K. A. D’Amour, J. D. Chesnut, M. S. Rao, C. A. Blau and A. J. Robins. Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling. Blood 2007 Dec 1;110(12):4111-9. doi: 10.1182/blood-2007-03-082586

[4] A. Youssef, D. Aboalola and V. K. Han. The Roles of Insulin-Like Growth Factors in Mesenchymal Stem Cell Niche. Stem Cells Int. (2017) 9453108. doi: 10.1155/2017/9453108

FAQ

IGF-1, also called somatomedin C, is a hormone with a similar molecular structure to insulin.

IGF-1 is primarily produced by the liver.

IGF-1 binds to the IGF-1 receptor (IGF1R)

IGF1R is linked to various cytoplasmic second messenger molecules including the RAS-MAPK and PI3K signaling networks.

IGF-1 can be used in ESC and MSC cultures.

Qkine high quality food grade products are intended solely for use as food processing aids, ex vivo cell manufacturing and research use. Not for direct human consumption, therapeutic or diagnostic use.

The authorization of novel foods, including the use of growth factors as food processing aids, is regulated by regional government agencies. The use of our products as food processing aids in novel foods requires the end-user to obtain the necessary regulatory approvals.

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