Recombinant bovine/porcine IGF-1 protein (Qk113-FG)£60.00 – £190.00View product
Recombinant bovine/porcine IGF-1 protein (Qk113-FG)
£60.00 – £190.00
Bovine/porcine insulin-like growth factor 1 (IGF-1) protein is used in the maintenance of induced pluripotent stem cells (iPSC) and is necessary for cell growth in the absence of insulin. IGF-1 promotes the proliferation of many cell types, including embryonic stem cells (ESC) and mesenchymal stem cells (MSC).
Qkine bovine/porcine IGF-1 is a high quality food grade 8 kDa protein, Qkine growth factors are animal origin-free (AOF) and carrier-protein free (CF) for consistent stem cell culture and cultured meat applications.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£60.00 – £190.00
1000µg will be despatched as 2 x 500µg
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Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High quality bovine/porcine IGF-1 protein (Uniprot: P07455)
- 8 kDa
High quality food grade recombinant protein
>98%, by SDS-PAGE quantitative densitometry
Animal origin-free (AOF) and carrier protein-free
Expressed in E. coli
Manufactured in the UK under a food manufacturing HACCP regime
Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Cell culture media optimization and replacement of insulin in defined media
Cellular agriculture and cultivated meat cell culture media optimization
Cellular agriculture process development
Frequently used together
Alternative proteins
IGF-1 activity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM). Cells are treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.
IGF-1 protein migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk047 batch #104331.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
Full raw materials traceability, allergen analysis, CoO, CoA, beta-lactam-free and animal origin-free certification available
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
The insulin-like growth factors (IGFs) are a family of proteins with a similar structure to insulin. IGF-1 has an A and B chain connected by disulphide bonds, its similarity to insulin means it can bind with low affinity to the insulin receptor [1]. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic, and lung [1]. IGF-1 exerts its biological effect through binding to the IGF1 receptor (IGF1R), its effect is modulated through interaction with a group of binding proteins (IGFBPs), which tend to inhibit the effect of IGF-1 [2]. Through IGF1R the Ras-MAPK and PI3 kinase signaling pathways are activated leading to increased cell proliferation and survival [2].
IGF-1 can be used to support maintenance of human embryonic stem cell (ESC) [3] and mesenchymal stem cell (MSC) cultures [3, 4].
Additional resources
FAQ
How is IGF-1 used in cell culture?
IGF-1 can be used in embryonic stem cell (ESC) and mesenchymal stem cell (MSC) cultures to promote proliferation, as a replacement to insulin.
What does IGF-1 bind to?
IGF-1 binds to the IGF-1 receptor (IGF1R)
Where is IGF-1 found?
IGF-1 is primarily produced by the liver.
What is IGF-1?
IGF-1, also called somatomedin C, is a hormone with a similar molecular structure to insulin.
What is the IGF-1 receptor pathway?
IGF1R is linked to various cytoplasmic second messenger molecules including the RAS-MAPK and PI3K signaling networks.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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