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IGF-1 LR3 bioactivity graph
Qk116-FG bpIGF-1 LR3 bioactivity
High quality food grade for cultivated meat, fish, fat and dairy

Recombinant bovine/porcine IGF-1 LR3 protein (Qk114-FG)

Bovine/porcine insulin-like growth factor 1 long arginine 3 (IGF-1 LR3) protein is a synthetic analog of IGF-1. The substitutions include an arginine substitution and an N-terminal protein extension. Consequently, IGF-1 LR3 has improved biological potency and extended half-life and can be used for the consistent, reproducible maintenance of pluripotent stem cells.

Qkine IGF-1 LR3 is a high quality food grade bioactive 9 kDa protein monomer, animal origin-free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg will be despatched as 2 x 500µg

Fast and free shipping.
Buy online with secure credit card or purchase order.
For any questions, please email orders@qkine.com

Summary

  • High quality IGF-1 LR3 protein

  • >98%, by SDS-PAGE quantitative densitometry

  • 9 kDa

  • Expressed in E. coli. 

  • Animal origin-free and carrier protein-free.

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from acetonitrile, TFA

  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Featured applications

  • High quality food grade recombinant protein

  • Cell culture media optimisation and replacement of insulin in defined media

Long R3 IGF-I, Insulin-like Growth Factor-I, Somatamedin C, IGF-IA

human
porcine (pig)
bovine (cow)

Frequently used together

Recombinant bovine/porcine FGF2-G3 145 aa protein (Qk080-FG)

Related discovery kits

B8 media discovery kit (Qk503)
Beefy9 media discovery kit (Qk504)
Serum-free media optimization kit (Qk505)

Bioactivity

IGF-1 LR3 bioactivity graph

Bioactivity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. Cells are treated in triplicate with a serial dilution of IGF-1 LR3 for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 1.3 nM (11.7 ng/ml). *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

IGF-1 LR3 bioactivity graph

IGF-1 LR3 migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R).  No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Analytical reversed-phase: single sharp peak 

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial >95%

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine.  To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

The insulin-like growth factors (IGFs) are a family of proteins with a similar structure to insulin. IGF-1 has an A and B chain connected by disulphide bonds, its similarity to insulin means it can bind with low affinity to the insulin receptor [1]. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic, and lung [1]. IGF-1 exerts its biological effect through binding to the IGF1 receptor (IGF1R), its effect is modulated through interaction with a group of binding proteins (IGFBPs), which tend to inhibit the effect of IGF-1 [2]. Through IGF1R the Ras-MAPK and PI3 kinase signaling pathways are activated leading to increased cell proliferation and survival [2].

IGF-1 can be used to support maintenance of human embryonic stem cell (ESC) [3] and mesenchymal stem cell (MSC) cultures [3, 4].

Insulin-like growth factor long arginine 3 (IGF-1 LR3), is a widely used synthetic analog of IGF-1 with an arginine replacing glutamic acid at position 3 (arginine 3, R3), and an additional 13 amino acids at its N-terminus (long, L). IGF-1 LR3 retains the ability to bind IGF-1 and insulin receptors but has reduced binding affinity to insulin-like growth factor-binding proteins (IGFBPs). Consequently, it has improved potency and metabolic stability [5].

[1] Z. Laron. Insulin-like growth factor 1 (IGF-1): a growth hormone. Mol Pathol. 2001 Oct;54(5):311-6. doi: 10.1136/mp.54.5.311

[2] H. Werner. The IGF1 Signaling Pathway: From Basic Concepts to Therapeutic Opportunities. International Journal of Molecular Sciences. 2023; 24(19):14882. doi.org/10.3390/ijms241914882

[3] L. Wang, T.C. Schulz, E. S. Sherrer, D. S. Dauphin, S. Shin, A. M. Nelson, C. B. Ware, M. Zhan, C. Z. Song, X. Chen, S. N. Brimble, A. McLean, M. J. Galeano, E. W. Uhl, K. A. D’Amour, J. D. Chesnut, M. S. Rao, C. A. Blau and A. J. Robins. Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling. Blood 2007 Dec 1;110(12):4111-9. doi: 10.1182/blood-2007-03-082586

[4] A. Youssef, D. Aboalola and V. K. Han. The Roles of Insulin-Like Growth Factors in Mesenchymal Stem Cell Niche. Stem Cells Int. (2017) 9453108. doi: 10.1155/2017/9453108

[5] F. M. Tomas, S. E. Knowles, P. C. Owens, C. S. Chandler, G. L. Francis, L. C. Read and F. J. Ballard. Insulin-like growth factor-I (IGF-I) and especially IGF-I variants are anabolic in dexamethasone-treated rats. Biochem J. 1992 Feb 15:282, 91-7. doi: 10.1042/bj2820091

FAQ

IGF-1 LR3 is a modified form of IGF-1, it is more potent and stable than unmodified IGF-1.

IGF-1 LR3 is a synthetic protein.

IGF-1 LR3 has an arginine instead of a glutamic acid at the third position in its amino acid sequence and an additional 13 amino acids at its N-terminus. IGF-1 LR3 remains an agonist of the IGF-1 receptor, but it has very low affinity for IGFBPs, which inhibit IGF-1, making it more potent. IGF-1 LR3 also has a significantly longer half-life than IGF-1.

IGF-1 LR3 binds to the IGF-1 receptor (IGF1R).

IGF1R is linked to various cytoplasmic second messenger molecules including the RAS-MAPK and PI3K signaling networks.

IGF-1 LR3 can be used to replace IGF-1 in stem cell cultures. Increased potency and stability means less IGF-1 LR3 is needed compared to unmodified IGF-1.

Qkine high quality food grade products are intended solely for use as food processing aids, ex vivo cell manufacturing and research use. Not for direct human consumption, therapeutic or diagnostic use.

The authorization of novel foods, including the use of growth factors as food processing aids, is regulated by regional government agencies. The use of our products as food processing aids in novel foods requires the end-user to obtain the necessary regulatory approvals.

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