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StemBeads Qkine EGF
StemBeads® Qkine EGF is a patented growth factor supplement that offers a novel way to culture cells with native EGF more efficiently, with greater control and fewer medium changes. StemBeads® Qkine EGF has been tested on neural progenitor cell (NPC) media, including neural progenitor expansion medium (NPEM), with enhanced cellular profiles.
StemBeads® revolutionary controlled-release protein technology combined with Qkine high purity and bioactivity animal origin-free growth factors and cytokines stabilizes growth factor levels for increased cell culture consistency and reproducibility.
Our robust animal origin-free manufacturing platform, along with rigorous quality control procedures, ensures exceptional bioactivity and consistent performance from lot to lot, guaranteeing outstanding performance in your applications.
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StemBeads® Qkine EGF is a patented growth factor supplement that offers a novel way to culture cells with native Endothelial Growth Factor more efficiently, with greater control and fewer medium changes. EGF is key for supporting differentiations. StemBeads® Qkine EGF are microbeads composed of an FDA approved, biodegradable PLGA polymer that is loaded with Qkine recombinant human EGF (Qk011) and release the encapsulated protein at a constant rate. Controlled delivery and stable levels (Figure 1) overcome the half-life of EGF and improve cell cultures while saving researchers valuable time and resources.
StemBeads are a revolutionary controlled-release growth factor technology. The StemBeads product is currently used for improved cell culture in more than 100 scientific laboratories globally. StemBeads technology encapsulates a growth factor in a PLGA hydrogel that stabilizes the protein and releases it steadily over several days. With growth factor levels stabilized, spontaneous differentiation is reduced, pluripotency is increased, and feeding schedules are reduced to only 2-3 times per week. StemBeads are added to your medium to achieve a desired growth factor release, sustained over several days. Following, the beads are washed from the culture, the medium is replaced, and the process repeated as required.
Members of the human epidermal growth factor family are synthesized as type I transmembrane precursor proteins, often containing several EGF domains in the extracellular region. Proteolysis yields mature proteins that are released from the cell surface [1]. Epidermal growth factor binds to high-affinity EGF receptors (EGFRs) and promotes receptor dimerization and clustering leading to activation of downstream signaling pathways, including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling [2]. Human epidermal growth factor (EGF) stimulates cell proliferation and differentiation. It is used extensively in induced-pluripotent stem cell (iPSC) and embryonic stem cell (ESC) culture systems for the successful expansion and differentiation of epithelial, neural, mesoderm and hematopoietic lineages [3]. EGF also stimulates the differentiation of mesenchymal stem cells (MSC) into bone-forming cells [4].
FAQ
StemBeads® can be easily removed prior to analysis by washing 1-2 times with PBS, DMEM, F12 or any other basal medium preferred. For suspension cultures, there are a variety of strainer pore sizes to allow for separation. Cells cultured with StemBeads® have been used in a variety of downstream applications, including but not limited to: immunofluorescence, FACS, RNA/DNA extraction, nucleofection, differentiation etc.
The protein concentration can be adjusted based on the number of StemBeads® that you use. The release rate has a linear correlation with the amount of StemBeads® used. For example, 8 µl of StemBeads® per ml of media releases at a concentration of 10 ng/ml. However, if you desire half of that concentration, such as 5 ng/ml, then adjust the concentration to 4 µl of StemBeads® per ml media to achieve the concentration of 5 ng/ml.
With growth factor levels stabilized, spontaneous differentiation is reduced, pluripotency is increased, and feeding schedules are reduced to only 2-3 times per week. StemBeads are added to your medium to achieve a desired growth factor release, sustained over several days. Following, the beads are washed from the culture, the medium is replaced, and the process repeated as required.
No, this is not a problem or indication of cell quality. Extensive testing has been performed to access the cell quality when using StemBeads®. All phenotypic and functional assays have shown cell quality as good or better than current feeding regimens. For best assessment of cells, wash cells once prior to observation.
Epidermal growth factor (EGF) is a growth factor which stimulates cell proliferation and differentiation.
Originally found in salivary glands and urine, EGF is known to be expressed in many human tissues, including platelets.
EGF primarily stimulates growth of epidermal and epithelial cells, but is also used for expansion of stem cells in feeder-free culture systems.
Epidermal growth factor receptor (EGFR) family are receptor tyrosine kinase (RTK) proteins. There are 4 members: EGFR (ErbB1, HER1), ErbB2 (HER2), ErbB3 (HER3) and ErbB4 (HER4).
Tyrosine phosphorylation of EGF receptors stimulates various signaling pathways, including Ras/MAPK, PLCγ1/PKC, PI3 kinase/Akt, and STAT pathways.
EGF can be added to many stem cell cultures to maintain them in their pluripotent state.
Qkine products are intended solely for research purposes and ex vivo cell manufacturing. Not for therapeutic or diagnostic use.
