Qkine activin A PLUS is as biologically active as activin A protein from an alternative supplier

Human/mouse/rat recombinant activin A PLUS protein is an optimised biologically active truncation of the mature domain of human activin A protein.  The EC50 and activity in stem cell culture of activin A PLUS is equivalent to the full mature domain activin A.

Qkine activin A PLUS (Qk005) is high-purity, animal origin-free, carrier protein-free and tag-free.

• Qkine activin A PLUS (Qk005) was bioactive in a quantitative luciferase assay with an EC50 of 6.9 pM.
• This was comparable to bioactivity of activin A from an alternative supplier, which had an EC50 of 7.9 pM.

Qkine activin A PLUS was as bioactive as activin A protein from an alternative supplier. Activin A activity was determined using the activin-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine activin A PLUS (Qk005, green) or Peprotech activin A (AF-120-14E, black). Protein concentrations were measured before use (Peprotech vial recovery 84%, Qkine 102%). Firefly luciferase activity was measured and normalized. Data from Qk005 lot #104344.

Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.