Technote | IL-7 (Qk095)

Qkine Interleukin 7 (IL-7) is as biologically active as a comparable alternative supplier protein

Interleukin-7 (IL-7) is a vital cytokine essential for immune system regulation, particularly in the development and maintenance of T cells, playing a crucial role in both adaptive and innate immune responses. Recombinant human IL-7 stimulates the development of lymphoid progenitor cells.

Qkine IL-7 (Qk095) manufacture has been optimized to produce a highly bioactive protein which is  animal origin-free, carrier protein-free and tag-free.

Qkine IL-7 bioactivity

  • Qkine IL-7 (Qk095) was bioactive in a quantitative assay with an EC50 of 0.41 ng/ml.
  • This was comparable to bioactivity of IL-7 standard from SBH Sciences, which had an EC50 of 0.44 ng/ml.

Qkine IL-7 was as bioactive as IL-7 from an alternative supplier. Recombinant IL-7 activity was determined using proliferation of the mouse-derived B lymphocyte cell line 2E8. Cells were treated in duplicate with a serial dilution of Qkine IL-7 (Qk095, green) for 65 hours. Cell viability was measured using the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (Promega). Assay was performed by SBH Sciences using their standard (black) for comparison. Data from Qk095 lot #204669.

Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of primary and stem cells.

All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.

About our products

Qkine_Lab_People_Pic0003-square-400x400

Nine-point Quality Commitment

Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.

ISO_square

Quality policy

Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.

carma800

Animal-free

Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.

Contact us

Our science team is here to help, please contact us if you have any questions.

Shopping Basket