Technote | IL-6 (Qk093)
Qkine interleukin-6 (IL-6) is stable and bioactive up to 28 days in cell culture conditions
Interleukin 6 is a multifunctional cytokine that regulates immune responses and inflammation. It is produced by various cells, including immune cells such as T cells and macrophages, as well as non-immune cells like fibroblasts and endothelial cells. IL-6 is used for the culture of hematopoietic stem cells (HSC) and other relevant cells.
Qkine IL-6 (Qk093) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine IL-6 stability
- After 14 days Qkine IL-6 (Qk093) bioactivity was equivalent to fresh IL-6, with an EC50 at day 0 of 3.2 ng/ml and 2.5 ng/ml at day 14.
- IL-6 was still bioactive after 28 days, with an EC50 at day 0 of 3.6 ng/ml and 3.5 ng/ml at day 28.
- No degradation of protein can be seen in an SDS-PAGE gel after 14 or 28 days.
Bioactivity and stability of Qkine IL-6. Bioactivity was determined using the IL-6-responsive firefly luciferase reporter assay. Transfected HEK293T cells were treated in triplicate for 24 hours with a serial dilution of IL-6 which had been pre-incubated in conditioned media for (A) 14 days or (B) 28 days. Firefly luciferase activity was measured and normalised to the control Renilla luciferase activity. (C) SDS-PAGE of recombinant IL-6 showed the protein was not degraded when incubated in HEK293T conditioned media for 14 or 28 days at 37°C.
Qkine IL-6 (Qk093) is stable and bioactive under cell culture conditions for up to 28 days. Qkine IL-6 is highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of myeloid cells and other relevant hematopoietic cells.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.