Technote | IL-6 (Qk093)
Qkine interleukin-6 (IL-6) is more biologically active than a comparable alternative supplier protein
Interleukin 6 is a multifunctional cytokine that regulates immune responses and inflammation. It is produced by various cells, including immune cells such as T cells and macrophages, as well as non-immune cells like fibroblasts and endothelial cells. IL-6 is used for the culture of hematopoietic stem cells (HSC) and other relevant cells.
Qkine IL-6 (Qk093) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine IL-6 bioactivity
- Qkine Interleukin 6 (Qk093) was bioactive in a quantitative luciferase assay with EC50 of 1.85 ng/ml (88.5 pM).
- This was more bioactive than Interleukin 6 from an alternative supplier, which had an EC50 of 5.74 ng/ml (274.6 pM).
Quantitative luciferase reporter assay shows Qkine IL-6 was more bioactive than an alternative supplier protein. HEK293T luciferase reporter cells were treated in triplicate with a serial dilution of Qkine IL-6 (Qk093, green) or an alternative supplier IL-6 (supplier B, black) for 24 hours. Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.
Qkine IL-6 (Qk093) was found to be more bioactive than IL-6 produced by an alternative major supplier. Qkine IL-6 has the advantage of being more cost effective, highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of HSC and other cells.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.