Technote | IL-3 (Qk090)
Qkine Interleukin 3 (IL-3) is as biologically active as the comparable alternative supplier protein
Interleukin 3 (IL-3) is a hematopoietic growth factor that promotes the proliferation, growth, and survival of myeloid cells. IL- 3 is commonly used in cell culture to stimulate the differentiation and maturation of human-induced pluripotent stem cells (iPSC) towards myeloid progenitors such as mast cells, basophils, neutrophils, eosinophils, monocytes, and megakaryocytes.
Qkine IL-3 (Qk090) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine IL-3 bioactivity
- Qkine IL-3 stimulated proliferation of TF-1 human myeloid leukemia cells with an EC50 of 108.7 pg/ml (7.2 pM).
- This was comparable to Supplier B bacterially expressed IL-3 bioactivity of 89.1 pg/ml (5.9 pM).
Qkine IL-3 has equivalent bioactivity to IL-3 from an alternative major supplier. Bioactivity was determined by stimulation of proliferation of TF-1 cells. Cells were treated in triplicate with a serial dilution of Qkine IL-3 (Qk090, green) or alternative supplier IL-3 (Supplier B black) for 72 hours and proliferation measured using the CellTiter-Glo (Promega) luminescence assay.
Qkine IL-3 (Qk090) has the advantage of being highly pure and animal origin-free, giving lot-lot consistency in bioactivity for long-term reproducible culture of myeloid cells and other relevant hematopoietic cells.
Download PDFAll our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.