Technote | IL-15 (Qk097)
Qkine Interleukin 15 (IL-15) is as biologically active as a comparable alternative supplier protein
Interleukin-15 (IL-15) is a pro-inflammatory cytokine which regulates immune responses. Recombinant IL-15 promotes the growth and activation of T-cells and B cells and is essential in the development, survival and activation of NK cells. IL-15 can also promote conversion of monocytes into dendritic cells.
Qkine has optimized the IL-15 manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility.
Qkine IL-15 bioactivity
- Qkine IL-15 (Qk097) was bioactive in a quantitative assay with an EC50 of 93 pg/ml (7.2 pM).
- This was comparable to the bioactivity of IL-15 from an alternative supplier, which had an EC50 of 97 pg/ml (7.5 pM).
Qkine IL-15 was as bioactive as IL-15 from an alternative supplier. Recombinant IL-15 activity was determined using the Promega IL-15 Bioassay Kit (JA2011). Cells were treated in triplicate with a serial dilution of Qkine IL-15 (Qk097, green) or Peprotech IL-15 (200-15, Supplier B, black) for 6 hours. Activity was measured using Bio-Gloâ„¢ Reagent and readings normalized to the maximum response. Data from Qk097 lot #204728.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of hematopoietic stem cells and immune cells.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
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