Technote | IGF-1 (Qk041)
Qkine IGF-1 LR3 is stable and bioactive up to 7 days in cell culture conditions
Human insulin-like growth factor 1 long arginine 3 (IGF-1 LR3) protein is a synthetic analog of IGF-1. The substitutions include an arginine substitution and an N-terminal protein extension. Consequently, IGF-1 LR3 has improved biological potency and extended half-life.
Recombinant IGF-1 LR3 can be used for the consistent, reproducible maintenance of human pluripotent stem cells.
Qkine IGF-1 LR3 stability
- After 7 days Qkine IGF-1 LR3 (Qk041) bioactivity was equivalent to fresh IGF-1 LR3, with an EC50 at day 0 of 15.5 ng/ml, day 2 EC50 14.6 ng/ml and day 7 EC50 23 ng/ml.
- No degradation of protein was seen in an SDS-PAGE gel after 2, 5 or 7 days.
Stability and bioactivity of Qkine IGF-1 LR3 (Qk041). SDS-PAGE of recombinant IGF-1 LR3 (A), protein was not degraded when incubated in HEK293T conditioned media for 7 days. Bioactivity was determined using the IGF-1 LR3-responsive firefly luciferase reporter assay (B). Transfected HEK293T cells were treated in triplicate for 4 hours with a serial dilution of IGF-1 LR3 which had been pre-incubated in conditioned media for 2 or 7 days. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity.
Qkine IGF-1 LR3 (Qk041) is stable and bioactive under cell culture conditions for up to 7 days. Qkine proteins highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of human pluripotent stem cells.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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