Technote | Vitronectin (Qk120)

Qkine recombinant vitronectin protein is ultra high-purity with exceptionally low residual endotoxins making it suitable for the reproducible culture of pluripotent stem cells, primary cells, organoids, and use in sensitive neural differentiations.

Preparation of cell culture plates with Qkine ultra-high quality vitronectin (Qk120)

• Briefly centrifuge vial containing vitronectin Qk120 to ensure all lyophilized protein is collected at the bottom of the vial.
• Resuspend in 500 ml of MilliQ water to make a 1 mg/ml stock and lightly agitate the tube to ensure everything has fully reconstituted.
• Per 6-well plate required, dilute 30 µl of the 1 mg/ml in 6 ml of phosphate buffered saline (PBS) without MgCl and CaCl to make 5 µg/ml solution.
• Coat each well of 6-well plate with 1 ml per well of 5 µg/ml vitronectin for at least two hours at 37°C.
• Adjust volume of 5 µg/ml vitronectin per well for different area plate clusters.
  • • 12-well plate add 500 µl
    • 24-well plate add 250 µl
    • 96-well plate add 100 µl
• Aliquot the remaining resuspended 1 mg/ml vitronectin into appropriately sized single use aliquots and store at -80°C.