Technote | FGF-8b (Qk057)
Qkine animal origin-free Fibroblast Growth Factor 8b produces consistently high bioactivity lot-to-lot
Fibroblast growth factor 8b (FGF-8b) is a spliced form of FGF–8, a member of the FGF family. FGFs regulate many developmental and cellular processes including proliferation, survival, angiogenesis and tumorigenesis. FGF-8b is involved in the regulation of embryogenesis and is commonly used for the differentiation of induced pluripotent stem cells (iPSC) into neural cell types and for brain organoid cultures.
Qkine FGF-8b (Qk057) is animal origin-free (AOF), carrier protein-free, tag-free and non-glycosylated to ensure high and consistent bioactivity.
Qkine FGF-8b lot-to-lot bioactivity validation
- Independent lots of FGF-8b (Qk057) were tested using a luciferase reporter assay in HEK293T cells.
- Qkine recombinant human FGF-8b lots showed consistent bioactivity in the range of 2.3-31 pg/ml (0.1-0.13 pM).
Qkine FGF-8b (Qk057) protein has exceptional lot-to-lot consistency. Bioactivity was determined using a firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of independent lots of FGF-8b for 3 hours in triplicate.
Qkine FGF-8b (Qk057) is highly pure, bioactive and animal origin-free. Qkine tests all newly manufactured lots against previous lots ensuring exceptional lot-to-lot consistency and allowing increased reproducibility for iPSC culture and differentiation.
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