Technote | Vitronectin (Qk121Qk121-FG)

Qkine recombinant bovine vitronectin protein is ultra high-purity with exceptionally low residual endotoxins making it suitable for the reproducible culture of pluripotent stem cells for cellular agriculture applications.

Preparation of cell culture plates with Qkine ultra-high quality bovine vitronectin (Qk121)

• Briefly centrifuge vial containing vitronectin Qk121 to ensure all lyophilized protein is collected at the bottom of the vial.
• Resuspend in 500 ml of MilliQ water to make a 1 mg/ml stock and lightly agitate the tube to ensure everything has fully reconstituted.
• Per 6-well plate required, dilute 30 µl of the 1 mg/ml in 6 ml of phosphate buffered saline (PBS) without MgCl and CaCl to make 5 µg/ml solution.
• Coat each well of 6-well plate with 1 ml per well of 5 µg/ml vitronectin for at least two hours at 37°C.
• Adjust volume of 5 µg/ml vitronectin per well for different area plate clusters.
  • • 12-well plate add 500 µl
    • 24-well plate add 250 µl
    • 96-well plate add 100 µl
• Aliquot the remaining resuspended 1 mg/ml vitronectin into appropriately sized single use aliquots and store at -80°C.