Technote | Activin E (Qk067)
Activin E PLUS™ Avi-tag has lower bioactivity than untagged Activin E PLUS™
Human recombinant Activin E PLUS™ (Avi-tag) protein is an optimized biologically active truncation of the mature domain of human Activin E protein with Avi-tag for easy biotinylation.
High purity 27.2 kDa dimer comprising truncated mature domain of activin E protein with an Avi-tag, animal origin-free (AOF) and carrier-protein free (CF).
Qkine activin E PLUS™ (Avi-tag) bioactivity
- Qkine activin E PLUS™ (Avi-tag) is an animal origin-free bioactive activin E with an EC50 of 1.0 ng/ml (37 pM).
- This is less bioactive than the untagged activin E PLUS™ which has an EC50 of 68 pg/ml (2.9 pM).
Recombinant activin E PLUS™ activity was determined using a Smad reporter assay in HEK293 cells co-transfected with the activin E co-receptor ALK7. Cells were treated in triplicate with a serial dilution of activin E PLUS™ (Qk067, black) or activin E PLUS™ (Avi-tag) protein (Qk082, green) for 6 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk082 lot #204688 and Qk062 lot #204646.
Although necessary for downstream assays the addition of an Avi-tag to activin E PLUS™ reduced its bioactivity. This highlights the importance of adding tags to proteins only when functional and needed.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible cell culture.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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