Qkine Interleukin 2 (IL-2) is as biologically active as a comparable alternative supplier protein

Interleukin-2 (IL-2) is a vital cytokine that regulates immune responses by promoting the growth and activation of T-cells, enhancing immune function, and maintaining immune tolerance for immune activation and suppression.

Qkine IL-2 (Qk089) manufacture has been optimized to produce a highly bioactive protein which is  animal origin-free, carrier protein-free and tag-free.

• Qkine IL-2 (Qk089) was bioactive in a quantitative assay with an EC50 of 49 pg/ml.
• This was comparable to bioactivity of IL-2 from an alternative supplier, which had an EC50 of 57 pg/ml.

Qkine IL-2 was as bioactive as IL-2 from an alternative supplier. Recombinant IL-2 activity was determined using the Promega IL-2 bioassay kit (JA2201). Cells were treated in triplicate with a serial dilution of Qkine IL-2 (Qk089, green) or Peprotech IL-2 (200-02-10UG, black) for 6 hours. Protein concentrations were measured before use (Peprotech vial recovery 105%, Qkine 96%). Activity was measured using Bio-Glo™ Reagent and readings normalised to the maximum response. Data from Qk089 lot #204686.

Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.