Qkine Insulin-like Growth Factor 2 (IGF-2) is as biologically active as a comparable alternative supplier protein

Insulin-like Growth Factor 2 (IGF-2) is a growth factor that promotes cell proliferation, differentiation, and survival, particularly during fetal development. IGF-2 also regulates tissue growth and muscle regeneration. 

Qkine IGF-2 (Qk118) manufacture has been optimized to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility.

• Qkine IGF-1 (Qk118) was bioactive in a quantitative assay with an EC50 of 58 ng/ml.
• This was comparable to bioactivity of IGF-2 from an alternative supplier, which had an EC50 of 50 ng/ml.

Qkine IGF-2 was as bioactive as IGF-2 from an alternative supplier. Bioactivity was determined using the serum response element (SRE) firefly luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of Qkine IGF-2 (Qk118, green) or Peprotech IGF-2 (100-12-10UG, black). Protein concentrations were measured before use (Peprotech vial recovery 99%, Qkine 109%). Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk118 lot #204687.

Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.