Technote | IGF-2 (Qk118)
Qkine insulin-like growth factor 2 (IGF-2) is as biologically active as a comparable alternative supplier protein
Insulin-like Growth Factor 2 (IGF-2) is a growth factor that promotes cell proliferation, differentiation, and survival, particularly during fetal development. IGF-2 also regulates tissue growth and muscle regeneration.Â
Qkine IGF-2 (Qk118) manufacture has been optimized to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility.
Qkine IGF-2 bioactivity
- Qkine IGF-1 (Qk118) was bioactive in a quantitative assay with an EC50 of 58 ng/ml.
- This was comparable to bioactivity of IGF-2 from an alternative supplier, which had an EC50 of 50 ng/ml.
Qkine IGF-2 was as bioactive as IGF-2 from an alternative supplier. Bioactivity was determined using the serum response element (SRE) firefly luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of Qkine IGF-2 (Qk118, green) or Peprotech IGF-2 (100-12-10UG, black). Protein concentrations were measured before use (Peprotech vial recovery 99%, Qkine 109%). Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk118 lot #204687.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
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