The bioactivity of Qk063 CNTF and bacterial-expressed CNTF from an alternative supplier was determined using the CNTF-responsive firefly luciferase reporter assay. HEK293T cells were treated in triplicate with a serial dilution of CNTF overnight. Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.
The bioactivity comparison demonstrates that Qkine CNTF has a 3-fold lower EC50 when compared to a major alternative supplier in our reporter assay. Qkine CNTF is highly pure, potent, and reliable for reproducible neural cell culture and CNTF-dependent applications.
Qkine CNTF and all Qkine recombinant proteins come with a Bioactivity Guarantee, which guarantees our proteins to be reproducibly bioactive in your cultures.