Technote | CNTF (Qk063)
Qkine ciliary neurotrophic factor (CNTF) is more biologically active than a comparable alternative supplier protein
Ciliary neurotrophic factor (CNTF) is a member of the IL-6 family of cytokines. CNTF protein plays a crucial role in regulating the development of the nervous system and is involved in the differentiation and maintenance of various neurons, glial cells, and retinal cells.
Qkine CNTF (Qk063) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine CNTF bioactivity
- The bioactivity of CNTF (Qk063) was determined to have an EC50 of 1.57 ng/ml (69 pM) using a CNTF-responsive firefly luciferase reporter assay.
- Qkine CNTF was 3-fold more bioactive than CNTF from Supplier 1 which had an EC50 of 5.29 ng/ml (232 pM).
Qkine CNTF was found to be more bioactive than CNTF from an alternative major supplier. Bioactivity was determined using a CNTF-response luciferase reporter assay in HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine CNTF (Qk063, green) or Peprotech (450-13, Supplier B, black) for 24 hours. Protein concentrations were measured before use (Peprotech recovery 102%, Qkine 103%). Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.
Qkine CNTF had higher bioactivity than CNTF from an alternative major supplier. Qkine CNTF (Qk063) has the advantage of being highly pure and animal origin-free, with increased bioactivity for cost-effective reproducible differentiation and maintenance of neural linages.
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