R-spondin 12019-05-30T08:30:38+00:00

Recombinant human R-spondin 1 (RSPO1)

R-spondin 1 (RSPO 1) is a secreted activator of the canonical Wnt signaling pathway.  A ligand for the leucine-rich repeat-containing G-protein coupled receptors (LGR) 4-61 , RSPO 1 is used extensively in adult stem cell-derived organoid culture, intestinal organoid culture, hematopoietic stem cell specification and other iPSC and culture systems1–5.

Qkine RSPO1 protein is a highly pure biologically active protein fragment comprising two cysteine-rich, furin-like domains, the essential domains for activity in stem cell culture.  Our E.coli-produced RSPO 1 is not glycosylated, which ensures reliable production of homogeneous protein and enhanced comparability between batches.  The glycosylation of R-spondin 1 is not necessary for extracellular signalling and stability 6.

Alternative protein names: roof plate-specific spondin-1, Rspo1, R-spondin-1, Cristin 3
Structure of R-spondin 1 cytokine

Summary: receptor-binding active domain of human R-spondin 1 (Uniprot: Q2MKA7) comprising two furin-like domains expressed in E.coli and purified to homogeneity.

Form: protein is provided in lyophilised form without carrier protein.

Molecular mass: 12.5 kDa

Quality testing: all our proteins are made in-house by our scientists.  We take the quality of our proteins very seriously and you can view the full quality testing data for each batch of protein by clicking on the link below

Qk006 RSPO1 batch #010

Handling guidance for lyophilised cytokines

Every effort is made to ensure samples are sterile however we recommend sterile filtering after dilution in media or the final working solution.

Buy online or email orders@qkine.com

We take the quality of our proteins very seriously

All our proteins are produced in-house by our scientists and we understand the impact on your work if your growth factors and cytokines don't perform as expected.

Please visit this article by our founder, Marko Hyvonen to understand why we think improving growth factor quality will save valuable time and expense.

Qkine quality promise: our growth factors and cytokines work. If they don’t, we give you your money back. Simple as that.

Find out more

Buy online or email orders@qkine.com