Recombinant human RSPO1 (Qk006) – beta launch!

£140.00£2,400.00

Receptor-binding active domain of human R-spondin 1 (Uniprot: Q2MKA7) comprising two furin-like domains expressed in E.coli and purified to homogeneity.

Datasheets

Batch-specific datasheets

SKU: Qk006_hRSPO1 Category: Tags: , , , , , ,

Description

***Beta launch*** we have developed an RSPO1 produced in E.coli and optimised for batch-batch consistency.  Our RSPO1 gives excellent results in the Top-Flash Wnt bioassay.  For a short-time only, we are launching this as a beta product as we gather more data on stem cell and organoid cultures.  During this period you can buy RSPO1 for 50% of the full cost.  If you send feedback (positive or negative) that you are happy for us to share with other scientists, we’ll also give you a free 100 ug unit of any of our growth factors.   Please email orders@qkine.com or order online.

R-spondin 1 (Rspo 1) is a secreted activator of the canonical Wnt signaling pathway.  It is a ligand for the leucine-rich repeat-containing G-protein coupled receptors (LGR) 4-61.  Rspo 1 is used extensively in adult stem cell-derived organoid culture, hematopoietic stem cell specification and other iPSC and culture systems1–5.

The Qkine protein is a highly pure biologically active protein fragment comprising the two cysteine-rich, furin-like domains, the essential domains for activity in stem cell culture.

We produce Rspo 1 in E.coli, which leads to lack of N-glycosylation.  The glycosylation of R-spondin 1 is unnecessary for extracellular signalling and stability 6.  Our methods allow for optimised reliable production of homogeneous protein and enhanced comparability between batches.

Product: recombinant human RSPO1 (Qk006)

Receptor-binding active domain of human R-spondin 1 (Uniprot: Q2MKA7) comprising two furin-like domains expressed in E.coli and purified to homogeneity.

Alternative names:

Roof plate-specific spondin-1, Rspo1, R-spondin-1, Cristin 3

Quality control:
We take the quality of our products very seriously, please see this blog by our founder, Marko Hyvonen to understand why we think improving growth factor quality will save valuable R&D time and expense. All our proteins are produced in-house by our scientists and we understand the potential impact on your work if your growth factors and cytokines are not consistent and of the highest quality.  We provide quality control data for each individual batch, please contact us any time if you have any questions.

The biochemical identity of each batch of human RSPO1 is testing by SDS-PAGE and mass spec.  Correct recovery from the vial is checked to ensure you will get what you expect.

Qkine quality promise: our growth factors and cytokines work. If they don’t, we give you your money back. Simple as that

Endotoxin testing:
Stem cell cultures are sensitive to endotoxins, which can be present in media, serum and as a contaminants on plastic ware. We optimise our protein production processes to ensure the lowest possible levels of endotoxin contamination.  Endotoxin levels are determined by an external expert microbiological testing services provider and we set our endotoxin pass criteria at the industry leading <0.1 EU per ug protein and aim for <0.01 EU per ug protein.  We report the exact endotoxin levels for each batch.

RSPO1 batch #010 endotoxin level = <0.0005 EU/ug protein (below the detection level of the assay) 

Please contact Meena at orders@qkine.com for batch reservation, bulk purchasing arrangements or any other questions.

References:

  1. de Lau, W. et al. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476, 293–297 (2011).
  2. de Lau, W., Peng, W. C., Gros, P. & Clevers, H. The R-spondin/Lgr5/Rnf43 module: regulator of Wnt signal strength. Genes Dev. 28, 305–316 (2014).
  3. Genthe, J. R. & Clements, W. K. R-spondin 1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways. Development 144, 590–600 (2017).
  4. Broutier, L. et al. Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat. Protoc. 11, 1724–1743 (2016).
  5. Broutier, L. et al. Human primary liver cancer–derived organoid cultures for disease modeling and drug screening. Nat. Med. 23, 1424–1435 (2017).
  6. Chang, C.-F. et al. N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability. doi:10.3390/ijms17060937

Additional information

Unit size

1000 µg, 500 µg, 100 µg, 50 µg, 25 µg

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