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  • Human/bovine/porcine TGF-β1 PLUS protein is the first entirely animal-free recombinant human transforming growth factor beta 1 (TGF-β1) protein for highly reproducible results and compatible with chemically-defined stem cell media. TGFβ1 is used extensively in E8-style induced pluripotent (iPSC) and embryonic (ESC) stem cell media. High purity 24 kDa dimer comprising optimised mature domain of TGFβ1 protein, animal-free (AF) and carrier-protein free (CF).  Our  TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.

    *Benchmark our TGF-β1 PLUS against your existing supplier*

  • Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems. 17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.
  • Recombinant R-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor.  In epithelial tissues LGR5 marks the stem cell population.  This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population.  R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells.  This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).
  • Human R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signalling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival. 13kDa highly pure, bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture.  Qk006 is animal-free and not glycosylated to ensure production of homogeneous protein and enhanced comparability between batches. Used to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.
  • Human OSM (oncostatin M) protein is used in the differentiation of human pluripotent stem cells into hepatocyte-like cells. OSM also plays roles in osteogenesis and neurogenesis, and is an important regulator of the hematopoietic stem cell niche in the bone marrow. Qkine human oncostatin M protein is high purity and animal and carrier-protein free for reproducible results.
  • Mouse/rat noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages. Qkine recombinant mouse noggin protein is a highly pure 46kDa bioactive dimer for reproducible results (animal-free and carrier-protein free).
  • Human noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages. Qkine human noggin protein is a highly pure 46kDa bioactive dimer for reproducible results in organoid culture.
  • Human NRG-1 (Neuregulin 1) protein is frequently used in the maintenance of human pluripotent stem cells.  In addition to its widespread use in stem cell culture media, NRG-1 (also known as Heregulin-β1 , HRG-1) has essential roles in vivo including in nervous system, cardiac, and mammary gland development; cancer biology and neurological disorders. 7.5 kDa highly pure, bioactive domain of human NRG-1, comprised of the β isoform of the EGF-like domain of NRG-1 (HRG1-B1). This NRG-1 protein monomer is animal-free (AF) and carrier-protein free (CF).
  • Mouse LIF (murine leukemia inhibitory factor) protein maintains the pluripotency and self-renewal of mouse  embryonic and induced pluripotent stem cells. Qkine recombinant mouse LIF protein is animal-free and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression.1
  • Human LIF (leukemia inhibitory factor) protein suppresses the differentiation of murine embryonic stem cells (ESCs) and is widely used in ESC and iPSC culture.1 Qkine human LIF protein is high purity and animal and carrier-protein free for reproducible results.
  • Human IGF-1 (IGF-I), insulin-like growth factor 1, protein is used in the maintenance of human pluripotent stem cells and is necessary for cell growth in the absence of insulin1. IGF-1 promotes the proliferation of many cell types, including mesenchymal stem cells, in vitro. Highly pure and bioactive 8 kDa IGF-1 protein, animal-free (AF) and carrier-protein free (CF)
  • Human IGF-1 LR3, insulin-like growth factor long arginine 3, protein is a synthetic form of IGF-1 with an N-terminal protein extension that improves potency and metabolic stability. IGF-1 LR3 is often used in the maintenance of human pluripotent stem cells1 Highly pure and bioactive 9 kDa IGR-1 LR3 protein monomer, animal-free (AF) and carrier-protein free (CF)
  • Human/bovine/porcine Gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to Noggin for optimisation of intestinal organoid culture and iPSC differentiation. Qk015 has been optimized by our experts for exceptionally high-purity production in E.coli and bioactivity. 18 kDa high purity dimeric protein, animal-free and carrier protein free
  • Human/rat/porcine/bovine FGF10 protein promotes lung organoid formation and induces branching morphology.  FGF10 protein is used widely in organoid culture, embryonic stem cell (ESC) and induced-pluripotent stem cell (iPSC) differentiation, and for the study of epithelial to mesenchymal transition and tumor metastasis. High purity and bioactivity 17 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 10, animal-derived component free (ACDF) and carrier-protein free (CF).
  • Human FGF-4 protein is used for the proliferation and differentiation of embryonic and induced-pluripotent and tissue (mesenchymal) stem cells and promotes neural stem cell proliferation.  FGF-4 is an important component of cardiac, intestinal and other organoid culture media. High purity and bioactivity 14 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 4, animal-derived component free (ACDF) and carrier-protein free (CF).
  • Zebrafish FGF-2 protein (bFGF/basic FGF) has been used extensively to support the maintenance and proliferation of human and mouse induced pluripotent (iPSC) and embryonic stem cells (ESC); used in the original Ludwig et al feeder-free culture of embryonic stem cells protocols 1-3. High purity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).  This version of recombinant zebrafish FGF2 is used by the core facility at the Cambridge Stem Cell Institute, UK and historically by many of the stem cell groups based at University of Cambridge.
  • mouse FGF2 / bFGF protein is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.1,2 High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).
  • Bovine/porcine FGF2 protein (bFGF/basic FGF) for development of species specific bovine (cow) and porcine (pig) cellular agriculture protocols and veterinary research applications. FGF-2 is used extensively in maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC)1-3  and for enhancement of proliferation in primary cell culture. High purity 16 kDa FGF-2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).
  • Human FGF-2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein.   FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF-2 comprises the core structured region and N-terminal extension. High purity and bioactivity 17 kDa FGF-2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).
  • Human FGF2 /bFGF protein (145 aa) is our gold-standard and recommended FGF2 / bFGF protein for induced pluripotent stem cell (iPSC) and embryonic stem cells (ESC) maintenance, and induced pluripotent and mesenchymal stem cells proliferation and differentiation. High purity and bioactivity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF) comprising the core structured region (145 amino acids) sufficient for full biological activity.

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