• Human EGF protein is a potent EGF-family growth factor used in many human and mouse organoid and stem cell culture systems including intestinal and tumor (cancer) organoid culture.A pure 6.3 kDa biologically active mature human epidermal growth factor. Our recombinant EGF protein is manufactured to the highest purity in E. coli and extensively tested, making it ideal for use in chemically defined iPSC, ESC and organoid culture media.
  • Human IGF-1 LR3, insulin-like growth factor long arginine 3, protein is a synthetic analog of IGF-1. The substitutions include an Arginine substitution and an N-terminal protein extension. Consequently, IGF-1 LR3 has improved biological potency and extended half-life.Highly pure and bioactive 9 kDa IGF-1 LR3 protein monomer, animal-free (AF) and carrier-protein free (CF). IGF-1 LR3 is often used in the maintenance of human pluripotent stem cells [1].
  • Human IGF-1 protein (IGF-I), insulin-like growth factor 1, is used in the maintenance of human pluripotent stem cells and is necessary for cell growth in the absence of insulin1. IGF-1 promotes the proliferation of many cell types, including mesenchymal stem cells, in vitro.Highly pure and bioactive 8 kDa IGF-1 protein, animal-free (AF) and carrier-protein free (CF)
  • Zebrafish FGF-2 protein (bFGF/basic FGF) has been used extensively to support the maintenance and proliferation of human and mouse induced pluripotent (iPSC) and embryonic stem cells (ESC); used in the original Ludwig et al feeder-free culture of embryonic stem cells protocols 1-3. High purity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).  This version of recombinant zebrafish FGF2 is used by the core facility at the Cambridge Stem Cell Institute, UK and historically by many of the stem cell groups based at University of Cambridge.
  • Human FGF2 /bFGF protein (145 aa) is our gold-standard and recommended FGF2 / bFGF protein for induced pluripotent stem cell (iPSC) and embryonic stem cells (ESC) maintenance, and induced pluripotent and mesenchymal stem cells proliferation and differentiation.High purity and bioactivity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF) comprising the core structured region (145 amino acids) sufficient for full biological activity.
  • Recombinant human FGF2 protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein. FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF-2 comprises the core structured region and N-terminal extension.High purity and bioactivity 17 kDa FGF-2 / bFGF protein, animal-free (AOF) and carrier-protein free (CF).
  • Recombinant bovine/porcine FGF2 protein 145 aa (bFGF/basic FGF) for the development of species-specific bovine (cow) and porcine (pig) cellular agriculture protocols and veterinary research applications. Used in comparative cell culture media optimization studies alongside Qk056, the 154aa form of porcine/bovine FGF-2.FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC)1-3  and for enhancement of proliferation in primary cell culture.High purity 16 kDa FGF-2 / bFGF protein, animal origin-free (AOF) and carrier-protein free (CF).
  • Recombinant mouse FGF2 protein/ bFGF is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.1,2High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).
  • Recombinant bovine/porcine FGF2 protein 154 aa (bFGF/basic FGF), this is the long form of FGF-2 used for the development of optimized serum-free culture media for species-specific bovine (cow) and porcine (pig) cultivated meat and veterinary research applications. Used in comparative media optimization studies with Qk040 porcine/bovine FGF-2 145 aa.FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC)1-3  and for enhancement of proliferation in primary cell culture.High purity 16 kDa FGF-2 / bFGF 154 aa protein, animal origin free (AOF) and carrier-protein free (CF).
  • Hepatocyte growth factor (HGF) is a multi-functional cytokine that promotes the growth and proliferation of various cell types including mesenchymal, endothelial, and epithelial cells. It is involved in embryonic development, cell motility, survival, angiogenesis, and tissue regeneration. In cell culture, HGF is used to promote the differentiation of mesenchymal cells towards hepatocytes, osteocytes, and endothelial cells. Recombinant human HFG NK1 protein is a potent naturally occurring isoform of HGF. This protein is animal-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency with a molecular weight of 20 kDa. It promotes efficient differentiation of human induced pluripotent stem cells to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α.  
  • Human/rat/porcine/bovine FGF10 protein promotes lung organoid formation and induces branching morphology.  FGF10 protein is used widely in organoid culture, embryonic stem cell (ESC) and induced-pluripotent stem cell (iPSC) differentiation, and for the study of epithelial to mesenchymal transition and tumor metastasis.High purity and bioactivity 17 kDa, bioactive domain of human fibroblast growth factor 10, animal-free (AOF) and carrier-protein free (CF).
  • Recombinant bovine HGF protein is a potent, high-purity NK1 isoform of bovine hepatocyte growth factor (HGF).   Bovine HGF differs from human HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding.  Use of species-specific HGF growth factor will facilitate media optimization for cellular agriculture (cultivated meat) and veterinary applications. Also available is Qk061 Porcine HGF (NK1). The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
  • Recombinant porcine HGF protein is a potent, high-purity NK1 isoform of porcine hepatocyte growth factor (HGF). Porcine Recombinant Protein HGF differs from human HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding. Use of species-specific HGF growth factor will facilitate media optimization for cellular agriculture (cultivated meat) and veterinary applications. Also available is Qk060 Bovine HGF (NK1). The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
  • Human/bovine/porcine Gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to Noggin for optimisation of intestinal organoid culture and iPSC differentiation.Qk015 has been optimized by our experts for exceptionally high-purity production in E.coli and bioactivity. 18 kDa high purity dimeric protein, animal-free and carrier protein free
  • Human NRG-1 protein (Neuregulin 1) is frequently used in the maintenance of human pluripotent stem cells.  In addition to its widespread use in stem cell culture media, NRG-1 (also known as Heregulin-β1 , HRG-1) has essential roles in vivo including in nervous system, cardiac, and mammary gland development; cancer biology and neurological disorders.7.5 kDa highly pure, bioactive domain of human NRG-1, comprised of the β isoform of the EGF-like domain of NRG-1 (HRG1-B1). This NRG-1 protein monomer is animal-free (AF) and carrier-protein free (CF).
  • FGF2-G3 (145 aa) protein is a thermostable engineered form of FGF-2 (bFGF). Qk052 comprises the 145 aa form of FGF-2 (Qk025) with the nine amino acid substitutions developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF2-G3 145 aa).FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. This new 145 aa thermostable FGF-2 protein allows direct comparison of efficacy in systems using FGF-2 145 aa (Qk025) for chemically defined stem cell and organoid culture media, and cultured meat media development.High purity 16 kDa bioactive FGF2-G3 145 aa protein. Animal-free (AOF), carrier protein-free and with no His-tag.
  • Recombinant FGF2-G3 (FGF2-STAB®) protein is a thermostable engineered form of FGF-2 (bFGF). Qk053 is the 154 aa mature domain of FGF-2 (Qk027) with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF2-G3).Recombinant FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. FGF2-G3 also has applications in chemically defined stem cell and organoid culture media, and cultured meat media development.High purity 17 kDa bioactive FGF2-G3 protein. Animal-free (AOF), carrier protein-free and with no His-tag.
  • Human/mouse/rat Activin A PLUS ™ protein is an optimised biologically active truncation of the mature domain of human Activin A protein.  The EC50 and activity in stem cell culture of activin A PLUS is equivalent to the full mature domain activin A.High purity 24 kDa dimer comprising truncated mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF).  Recombinant Activin A PLUS is designed to be manufactured at scale for cost-effective, large-scale stem cell culture applications.
  • R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signaling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.Recombinant human R-spondin 1 protein (RSPO1) protein is the bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1. Those are necessary and sufficient for Wnt signaling potentiation and are the essential domains for activity in stem cell and organoid culture.This protein has a molecular weight of 13 kDa and is animal-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk006 is suitable to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.
  • Mouse LIF (murine leukemia inhibitory factor) protein maintains the pluripotency and self-renewal of mouse  embryonic and induced pluripotent stem cells. Qkine recombinant mouse LIF protein is animal-free and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression.1

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