- Transforming growth factor-beta 1 (TGF-β1) is a pleiotropic cytokine that regulates various cellular processes, including cell proliferation, growth, differentiation, motility, and apoptosis. It is an essential growth factor in many embryonic and induced pluripotent stem cell maintenance media, including the commonly used E8, StemPro, and mTeSR media. TGF-β1 also promotes the differentiation of various cell types such as fibroblasts, epithelial cells, and immune cells.Human/bovine/porcine recombinant TGF-β1 PLUS protein is the first entirely animal-free recombinant human TGF-β1 protein for highly reproducible results and compatible with chemically-defined stem cell media.TGF-β1 PLUS is a high purity 24 kDa dimer comprising optimized mature domain of TGFβ1 protein, animal-free (AF) and carrier-protein free (CF). Our TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.*Benchmark our TGF-β1 PLUS against your existing supplier*
- Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.
- Recombinant human r-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor. In epithelial tissues LGR5 marks the stem cell population. This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population. R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells. This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).
- R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signaling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.Recombinant human R-spondin 1 protein (RSPO1) protein is the bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1. Those are necessary and sufficient for Wnt signaling potentiation and are the essential domains for activity in stem cell and organoid culture.This protein has a molecular weight of 13 kDa and is animal-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk006 is suitable to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.
- Human PDGF-BB (platelet derived growth factor BB) protein is a homodimer of PDGF-B peptide chains. PDGF is a potent mitogen involved in embryogenesis.1PDGF-BB is used in neuronal differentiation2 and treatment of periodontal and orthopedic bone defects and dermal wound healing.3High purity 25 kDa disulfide–linked bioactive dimeric human PDGF-B protein. Animal-free (AF) and carrier protein-free.
- Human PDGF-AA (platelet derived growth factor AA) protein is a homodimer of PDGF-A peptide chains. This protein is a potent mitogen involved in embryogenesis.1 PDGF-AA is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells.2High purity 29 kDa disulfide–linked bioactive dimeric human PDGF-A protein. Animal-free (AF) and carrier protein-free.
- Human OSM protein (oncostatin M) is used in the differentiation of human pluripotent stem cells into hepatocyte-like cells. OSM also plays roles in osteogenesis and neurogenesis, and is an important regulator of the hematopoietic stem cell niche in the bone marrow. Qkine human oncostatin M protein is high purity and animal and carrier-protein free for reproducible results.
- Mouse/rat noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.Qkine recombinant mouse noggin protein is a highly pure 46kDa bioactive dimer for reproducible results (animal-free and carrier-protein free).
- Human noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.Qkine human noggin protein is a highly pure 46kDa bioactive dimer for reproducible results in organoid culture.
- Human NRG-1 protein (Neuregulin 1) is frequently used in the maintenance of human pluripotent stem cells. In addition to its widespread use in stem cell culture media, NRG-1 (also known as Heregulin-β1 , HRG-1) has essential roles in vivo including in nervous system, cardiac, and mammary gland development; cancer biology and neurological disorders.7.5 kDa highly pure, bioactive domain of human NRG-1, comprised of the β isoform of the EGF-like domain of NRG-1 (HRG1-B1). This NRG-1 protein monomer is animal-free (AF) and carrier-protein free (CF).
- Human LIF (leukemia inhibitory factor) protein suppresses the differentiation of murine embryonic stem cells (ESCs) and is widely used in ESC and iPSC culture.1 Qkine human LIF protein is high purity and animal and carrier-protein free for reproducible results.
- Mouse LIF (murine leukemia inhibitory factor) protein maintains the pluripotency and self-renewal of mouse embryonic and induced pluripotent stem cells. Qkine recombinant mouse LIF protein is animal-free and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression.1
- Human IGF-1 LR3, insulin-like growth factor long arginine 3, protein is a synthetic analog of IGF-1. The substitutions include an Arginine substitution and an N-terminal protein extension. Consequently, IGF-1 LR3 has improved biological potency and extended half-life.Highly pure and bioactive 9 kDa IGF-1 LR3 protein monomer, animal-free (AF) and carrier-protein free (CF). IGF-1 LR3 is often used in the maintenance of human pluripotent stem cells [1].
- Human IGF-1 protein (IGF-I), insulin-like growth factor 1, is used in the maintenance of human pluripotent stem cells and is necessary for cell growth in the absence of insulin1. IGF-1 promotes the proliferation of many cell types, including mesenchymal stem cells, in vitro.Highly pure and bioactive 8 kDa IGF-1 protein, animal-free (AF) and carrier-protein free (CF)
- Recombinant porcine HGF protein is a potent, high-purity NK1 isoform of porcine hepatocyte growth factor (HGF). Porcine Recombinant Protein HGF differs from human HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding. Use of species-specific HGF growth factor will facilitate media optimization for cellular agriculture (cultivated meat) and veterinary applications. Also available is Qk060 Bovine HGF (NK1). The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
- Hepatocyte growth factor (HGF) is a multi-functional cytokine that promotes the growth and proliferation of various cell types including mesenchymal, endothelial, and epithelial cells. It is involved in embryonic development, cell motility, survival, angiogenesis, and tissue regeneration. In cell culture, HGF is used to promote the differentiation of mesenchymal cells towards hepatocytes, osteocytes, and endothelial cells. Recombinant human HFG NK1 protein is a potent naturally occurring isoform of HGF. This protein is animal-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency with a molecular weight of 20 kDa. It promotes efficient differentiation of human induced pluripotent stem cells to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α.
- Recombinant bovine HGF protein is a potent, high-purity NK1 isoform of bovine hepatocyte growth factor (HGF). Bovine HGF differs from human HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding. Use of species-specific HGF growth factor will facilitate media optimization for cellular agriculture (cultivated meat) and veterinary applications. Also available is Qk061 Porcine HGF (NK1). The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
- Human/bovine/porcine Gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to Noggin for optimisation of intestinal organoid culture and iPSC differentiation.Qk015 has been optimized by our experts for exceptionally high-purity production in E.coli and bioactivity. 18 kDa high purity dimeric protein, animal-free and carrier protein free
- Human/rat/porcine/bovine FGF10 protein promotes lung organoid formation and induces branching morphology. FGF10 protein is used widely in organoid culture, embryonic stem cell (ESC) and induced-pluripotent stem cell (iPSC) differentiation, and for the study of epithelial to mesenchymal transition and tumor metastasis.High purity and bioactivity 17 kDa, bioactive domain of human fibroblast growth factor 10, animal-free (AOF) and carrier-protein free (CF).
- Human FGF-4 protein is used for the proliferation and differentiation of embryonic and induced-pluripotent and tissue (mesenchymal) stem cells and promotes neural stem cell proliferation. FGF-4 is an important component of cardiac, intestinal and other organoid culture media.High purity and bioactivity 14 kDa, bioactive domain of human fibroblast growth factor 4, animal origin free (AOF) and carrier-protein free.
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