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    For current hiPSC media comparison with B8 media to see if your cells can be maintained as efficiently while giving you the weekends off.

     B8 hiPSC media, developed in the Burridge lab at Northwestern University, is growing in popularity thanks to its cost-effective and weekend-free cell culture credentials. B8 medium is a thermostable version of Essential 8 Medium. It is xeno-free and feeder-free medium for the growth and expansion of human pluripotent stem cells.The B8 media Discovery kit allows the optimization of the published B8 recipe, including modified versions with higher concentrations of TGF-β1/3 (1 or 2 ng/ml), to evaluate how concentrations affect the expression of pluripotency markers. Compare B8 media with current hiPSC media to evaluate whether your cells can be maintained as efficiently with weekend-free culture.
  • Recombinant FGF2-G3 (FGF2-STAB®) protein is a thermostable engineered form of FGF-2 (bFGF). Qk053 is the 154 aa mature domain of FGF-2 (Qk027) with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF2-G3).Recombinant FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. FGF2-G3 also has applications in chemically defined stem cell and organoid culture media, and cultured meat media development.High purity 17 kDa bioactive FGF2-G3 protein. Animal-free (AOF), carrier protein-free and with no His-tag.

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