Epidermal growth factor (EGF) recombinant protein for induced-pluripotent stem cell (iPSC), embryonic stem cell (ESC) and organoid culture.  For successful expansion and differentiation of epithelial, neural, mesoderm and hematopoietic lineages.  Use along with other stem cell niche factors, R-spondin 1, Noggin or Gremlin, Wnt3a and FGF10, in defined and animal-derived component free media for establishing and maintaining intestine, stomach, liver, pancreas, brain and cancer organoids.